Goat P53 Polyclonal Antibody | anti-P53 antibody

P53 Polyclonal Antibody

Cat. Num. AAA448112

• Gene Names: TP53; P53; BCC7; LFS1; TRP53
• Reactivity: Human, Mouse, Rat, Bovine, Canine, Chicken, Avian, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Porcine, Rabbit, Sheep, Simian
• Applications: Western Blot, Immunofluorescence
• Purity: Epitope Affinity Purified
• Synonyms: P53; Polyclonal Antibody; P53 Polyclonal Antibody; Anti-P53; BCC7; LFS1; TRP53 antibody; anti-P53 antibody

• Host: Goat
• Reactivity: Human, Mouse, Rat, Bovine, Canine, Chicken, Avian, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Porcine, Rabbit, Sheep, Simian
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of P53 by Western blot and in transfected cells with GFP-P53.
• Purity/Purification: Epitope Affinity Purified
• Form/Format: Polyclonal antibody supplied as a 200 ul (4 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
• Concentration: 4 mg/ml (varies by lot)
• Sequence Length: 393

• Applicable Applications for anti-P53 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Western blot: 1:500-1:5,000; Immunofluorescence: 1:25 - 1:250
• Immunogen: Recombinant peptide derived from within residues 280 aa to the C-terminus of human P53 produced in E Coli.
• Conjugation: Unconjugated
• Preparation and Storage: Long-term storage: Store at -20 degree C.; Short term storage: Store at 2-8 degree C for up to one month.

Reactivity Data

Testing Data

Testing Data

Related Product Information for anti-P53 antibody

Goat polyclonal antibody to P53. This protein reacts to diverse cellular stresses to regulate target genes that induce apoptosis, senescence, cell cycle arrest, DNA repair, or changes in metabolism. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is assumed to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumour suppressor.

NCBI and Uniprot Product Information

• NCBI GI #: 23491729
• NCBI GeneID: 7157
• Molecular Weight: 24,401 Da
• NCBI Official Full Name: P53
• NCBI Official Synonym Full Names: tumor protein p53
• NCBI Official Symbol: TP53
• NCBI Official Synonym Symbols: P53; BCC7; LFS1; TRP53
• NCBI Protein Information: cellular tumor antigen p53
• UniProt Protein Name: Cellular tumor antigen p53
• Protein Family: p53 and DNA damage-regulated protein
• UniProt Gene Name: TP53
• UniProt Synonym Gene Names: P53
• UniProt Entry Name: P53_HUMAN

Uniprot Description

p53: a transcription factor and major tumor suppressor that plays a major role in regulating cellular responses to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. More than 50 percent of human tumors contain a mutation or deletion of the TP53 gene. p53 is modified post-translationally at multiple sites. DNA damage induces phosphorylation of p53 at S15, S20 and S37, reducing its interaction with the oncoprotein MDM2. MDM2 inhibits p53 accumulation by targeting it for ubiquitination and proteasomal degradation. Phosphorylated by many kinases including Chk2 and Chk1 at S20, enhancing its tetramerization, stability and activity. The phosphorylation by CAK at S392 is increased in human tumors and has been reported to influence the growth suppressor function, DNA binding and transcriptional activation of p53. Phosphorylation of p53 at S46 regulates the ability of p53 to induce apoptosis. The acetylation of p53 appears to play a positive role in the accumulation of p53 during the stress response. Following DNA damage, p53 becomes acetylated at K382, enhancing its binding to DNA. Deacetylation of p53 can occur through interaction with SIRT1, a deacetylase that may be involved in cellular aging and the DNA damage response. p53 regulates the transcription of a set of genes encoding endosomal proteins that regulate endosomal functions. These include STEAP3 and CHMP4C, which enhance exosome production, and CAV1 and CHMP4C, which produce a more rapid endosomal clearance of the EGFR from the plasma membrane. DNA damage regulates a p53-mediated secretory pathway, increasing the secretion of some proteins such as Hsp90, SERPINE1, SERPINB5, NKEF-A, and CyPA, and inhibiting the secretion of others including CTSL and IGFBP-2. Two alternatively spliced human isoforms have been reported. Isoform 2 is expressed in quiescent lymphocytes. Seems to be non-functional. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Protein type: Activator; DNA-binding; Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Transcription factor; Tumor suppressor

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: cytoplasm; cytosol; mitochondrion; nuclear body; nuclear chromatin; nuclear matrix; nucleolus; nucleoplasm; nucleus; PML body; protein complex; replication fork; transcription factor TFIID complex

Molecular Function: ATP binding; chaperone binding; chromatin binding; copper ion binding; damaged DNA binding; DNA binding; double-stranded DNA binding; enzyme binding; histone acetyltransferase binding; identical protein binding; p53 binding; protease binding; protein binding; protein heterodimerization activity; protein kinase binding; protein N-terminus binding; protein phosphatase 2A binding; protein phosphatase binding; protein self-association; receptor tyrosine kinase binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding; ubiquitin protein ligase binding; zinc ion binding

Biological Process: base-excision repair; cell aging; cell cycle arrest; cell differentiation; cell proliferation; cellular response to glucose starvation; chromatin assembly; circadian behavior; determination of adult life span; DNA damage response, signal transduction by p53 class mediator; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; DNA strand renaturation; entrainment of circadian clock by photoperiod; ER overload response; G1 DNA damage checkpoint; multicellular organismal development; negative regulation of apoptosis; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of fibroblast proliferation; negative regulation of helicase activity; negative regulation of telomerase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; nucleotide-excision repair; positive regulation of apoptosis; positive regulation of histone deacetylation; positive regulation of neuron apoptosis; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein export from nucleus; positive regulation of protein oligomerization; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; proteasomal ubiquitin-dependent protein catabolic process; protein complex assembly; protein localization; protein sumoylation; protein tetramerization; Ras protein signal transduction; regulation of apoptosis; regulation of mitochondrial membrane permeability; regulation of transcription, DNA-dependent; response to antibiotic; response to DNA damage stimulus; response to gamma radiation; response to X-ray; viral reproduction

Disease: Adrenocortical Carcinoma, Hereditary; Basal Cell Carcinoma, Susceptibility To, 7; Breast Cancer; Colorectal Cancer; Glioma Susceptibility 1; Hepatocellular Carcinoma; Li-fraumeni Syndrome 1; Nasopharyngeal Carcinoma; Osteogenic Sarcoma; Pancreatic Cancer; Papilloma Of Choroid Plexus