Rabbit p53 Polyclonal Antibody | anti-p53 antibody

p53 antibody

Cat. Num. AAA5309697

• Gene Names: TP53; P53; BCC7; LFS1; TRP53
• Applications: Western Blot
• Purity: Purified by immunoaffinity chromatography.
• Synonyms: p53; Polyclonal Antibody; p53 antibody; Rabbit polyclonal p53 antibody; Polyclonal p53 antibody; Anti-p53 antibody; p 53 antibody; p-53 antibody; Ab 46 antibody; p-53; p53 Oncoprotein antibody; p 53; tumor suppressing protein antibody; tumor suppressor homolog p53 antibody; anti-p53 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Purity/Purification: Purified by immunoaffinity chromatography.
• Form/Format: Liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl,0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 393

• Applicable Applications for anti-p53 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: p53 antibody was raised in Rabbit using the peptide sequence around aa 44~48 (M-L-S-P-D) derived from Human p53 as the immunogen.
• Cross-Reactivity: Human
• Preparation and Storage: Store at 4 degree C for short term storage.; Aliquot and store at-20 degree C for long term storage.; Avoid repeated freeze/thaw cycles.

Testing Data

(Western Blot analysis of extracts from HT29 cells using p53 antibody)

Related Product Information for anti-p53 antibody

The p53 gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism.

Product Categories/Family for anti-p53 antibody

DNA & RNA

NCBI and Uniprot Product Information

• NCBI GI #: 23491729
• NCBI GeneID: 7157
• Molecular Weight: 24,401 Da
• NCBI Official Full Name: P53
• NCBI Official Synonym Full Names: tumor protein p53
• NCBI Official Symbol: TP53
• NCBI Official Synonym Symbols: P53; BCC7; LFS1; TRP53
• NCBI Protein Information: cellular tumor antigen p53
• UniProt Protein Name: Cellular tumor antigen p53
• Protein Family: p53 and DNA damage-regulated protein
• UniProt Gene Name: TP53
• UniProt Synonym Gene Names: P53

NCBI Description

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]

Uniprot Description

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).