Mouse p21WAF1 Monoclonal Antibody | anti-p21WAF1 antibody

p21WAF1 (Tumor Suppressor Protein)

Cat. Num. AAA439835

• Gene Names: CDKN1A; P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
• Reactivity: Human.; Does not react with Mouse and Rat. Others not known.
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunofluorescence
• Synonyms: p21WAF1; Monoclonal Antibody; p21WAF1 (Tumor Suppressor Protein); Activating Fragment 1; CAP20; CDK-interacting protein 1; CDKI; CDKN1; CDKN1A; CIP1; Cyclin-dependent kinase inhibitor 1A (p21; Cip1); DNA Synthesis Inhibitor; MDA6; Melanoma Differentiation Associated Protein 6; p21Cip1/Waf1; PIC1; SDI1; SLC12A9; Wild type p53 activated fragment 1 (WAF1); anti-p21WAF1 antibody

• Host: Mouse
• Reactivity: Human.; Does not react with Mouse and Rat. Others not known.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: AC8
• Specificity: This MAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdks and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 164

• Applicable Applications for anti-p21WAF1 antibody: ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
• Application Notes: ELISA: For coating use Ab at 1-5ug/ml, order Ab without BSA.; WB: 0.5-1ug/ml; IP: 1-2ug/500ug protein; IF: 1-2ug/ml
• Immunogen: Recombinant full-length human CDKN1A protein
• Cellular Localization: Nuclear
• Hu-chromosome Location: 6p21.31
• Positive Control: HeLa or MCF7 cells.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-Page

(SDS-PAGE Analysis Purified p21 Mouse Monoclonal Antibody (AC8). Confirmation of Integrity and Purity of Antibody.)

SDS-Page

(SDS-PAGE Analysis Purified AKT1 Mouse Monoclonal Antibody (AKT1/2552). Confirmation of Integrity and Purity of Antibody.)

References

Harper, J.W., et al. 1993. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 75: 805-816.

NCBI and Uniprot Product Information

• NCBI GI #: 11386203
• NCBI GeneID: 1026
• NCBI Accession #: NP_000380.1
• NCBI GenBank Nucleotide #: NP_000380.1
• UniProt Accession #: P38936; Q14010; Q6FI05; Q9BUT4
• Molecular Weight: 21kDa
• NCBI Official Full Name: cyclin-dependent kinase inhibitor 1 isoform 1
• NCBI Official Synonym Full Names: cyclin dependent kinase inhibitor 1A
• NCBI Official Symbol: CDKN1A
• NCBI Official Synonym Symbols: P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
• NCBI Protein Information: cyclin-dependent kinase inhibitor 1
• UniProt Protein Name: Cyclin-dependent kinase inhibitor 1
• UniProt Gene Name: CDKN1A
• UniProt Synonym Gene Names: CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; MDA-6

NCBI Description

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (PubMed:11595739).