Mouse anti-Human CD84 / SLAMF5 Monoclonal Antibody | anti-CD84 antibody

CD84 / SLAMF5 Mouse Monoclonal Antibody

Cat. Num. AAA438874

• Gene Names: CD84; LY9B; hCD84; mCD84; SLAMF5
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: CD84 / SLAMF5; Monoclonal Antibody; CD84 / SLAMF5 Mouse Monoclonal Antibody; Cell surface antigen MAX.3; hCD84; Hly9-beta; Leucocyte differentiation antigen CD84; LY9B; Signaling lymphocytic activation molecule 5 (SLAMF5); SLAM family member 5; anti-CD84 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: 152-1D5
• Specificity: Recognizes a protein of 74kDa, identified as CD84 (Workshop V; Code A085, C057). It is expressed on mature B cells and on B-cell lines, including pre-B-cell lines, but not on plasma cell lines. Immunohistochemical studies demonstrated that CD84 strongly expressed on tissues macrophages. CD84 is also highly expressed on platelets and, at low levels on peripheral blood T cells. It is a highly N-glycosylated protein and belongs to immunoglobulin superfamily. It may play a role in leukocyte activation.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 345

• Applicable Applications for anti-CD84 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml)
• Cellular Localization: Cell Surface
• Immunogen: Spleen cells of a patient with hairy cell leukemia
• Hu-Chromosome Location: 1q24
• Positive Control: Raji, HL60, THP-1, HMC-1, HEL and NAMALWA cells. Human platelets and macrophages. Human tonsil.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

References

Schlossman SF et al. (eds) Leukocyte Typing V. p.699-700, Oxford University Press, Oxford, 1995. de la Fuente MA et al. CD84 leukocyte antigen is a new member of the Ig superfamily. Blood 1997, 90(6):2398-2405. Kishimoto T. et al., eds. Leukocyte Typing VI, Garland Publishing, Inc, New York, 1997

NCBI and Uniprot Product Information

• NCBI GI #: 296434285
• NCBI GeneID: 8832
• NCBI Accession #: NP_001171808.1
• NCBI GenBank Nucleotide #: NM_001184879.1
• UniProt Accession #: Q9UIB8; O15430; O95266; O95660; Q5H9R1; Q6FHA8; Q8WLP1; Q8WWI8; Q9UF04; Q9UIB6; B2R8T1; B7Z3R8
• Molecular Weight: 74kDa
• NCBI Official Full Name: SLAM family member 5 isoform 1
• NCBI Official Synonym Full Names: CD84 molecule
• NCBI Official Symbol: CD84
• NCBI Official Synonym Symbols: LY9B; hCD84; mCD84; SLAMF5
• NCBI Protein Information: SLAM family member 5
• UniProt Protein Name: SLAM family member 5
• Protein Family: SLAM family
• UniProt Gene Name: CD84
• UniProt Synonym Gene Names: SLAMF5
• UniProt Entry Name: SLAF5_HUMAN

NCBI Description

This gene encodes a membrane glycoprotein that is a member of the signaling lymphocyte activation molecule (SLAM) family. This family forms a subset of the larger CD2 cell-surface receptor Ig superfamily. The encoded protein is a homophilic adhesion molecule that is expressed in numerous immune cells types and is involved in regulating receptor-mediated signaling in those cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2011]

Uniprot Description

CD84: Plays a role as adhesion receptor functioning by homophilic interactions and by clustering. Recruits SH2 domain- containing proteins SH2D1A/SAP. Increases proliferative responses of activated T-cells and SH2D1A/SAP does not seen be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A/SAP-dependent pathway. May serve as a marker for hematopoietic progenitor cells. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Motility/polarity/chemotaxis; Membrane protein, integral; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 1q24

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding

Biological Process: defense response; homophilic cell adhesion; leukocyte migration; negative regulation of granulocyte macrophage colony-stimulating factor production; negative regulation of interleukin-18 production; negative regulation of mast cell activation; negative regulation of mast cell degranulation