Mouse anti-Human. c-Myb Oncoprotein Monoclonal Antibody | anti-MYB antibody

c-Myb Oncoprotein

Cat. Num. AAA438289

• Gene Names: MYB; efg; Cmyb; c-myb; c-myb_CDS
• Reactivity: Human.
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry
• Synonyms: c-Myb Oncoprotein; Monoclonal Antibody; c-myb_CDS; efg; ONCOGENE AMV; Oncogene MYB; Proto-oncogene c-Myb; Transcriptional activator Myb; v-myb avian myeloblastosis viral oncogene homolog; anti-MYB antibody

• Host: Mouse
• Reactivity: Human.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: MYB286
• Specificity: The highly leukemogenic avian retrovirus E26 contains two oncogenes, v-Myb and v-Ets, which are expressed together as a fusion protein. The cellular hom- olog of v-Myb, designated c-Myb, encodes a transcription factor. Deletion or disruption of a negative regulatory domain mapping within the carboxy-ter- minal domain of c-Myb results in enhanced transactivating capacity and in parallel, leads to activation of its ability to transform hemopoietic cells. c-Myb is expressed preferentially, but not exclusively, in immature hemopoietic cells and its expression decreases as cells differentiate.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 637

• Applicable Applications for anti-MYB antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP), Immunohistology (IH)
• Application Notes: ELISA (For coating, order Ab without BSA); Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); Western Blotting (0.5-1ug/ml); Immunoprecipitation (1-2ug/500ug protein lysate); Optimal dilution for a specific application should be determined.
• Localization: Nuclear
• Positive Control: Jurkat, MOLT-4, COLO or BT-20 cells. Colon or breast carcinomas.
• Human Chromosomal Location: 6q23.3
• Immunogen: A synthetic peptide, corresponding to aa 119-135 (RRKVEQEGYPQESSKAG) of c-myb oncoprotein, coupled to KLH
• Quantity and Buffer: 0.5 ml at 200ug/ml with BSA and azide
• Preparation and Storage: Store at 2 to 8 degree C. Antibody is stable for 24 months.

References

1. Gonda, T.J., et al. 1984. Expression of Myb, Myc and Fos proto-oncogenes during the differentiation of a murine myeloid leukaemia. Nature 310: 249-251.

NCBI and Uniprot Product Information

• NCBI GI #: 194328727
• NCBI GeneID: 4602
• NCBI Accession #: NP_001123644.1
• NCBI GenBank Nucleotide #: NM_001130172.1
• UniProt Accession #: P10242; P78391; P78392; P78525; P78526; Q14023; Q14024; E9PI07; E9PLZ5; E9PNA4; E9PNL6; E9PRS2
• Molecular Weight: ~75kDa
• NCBI Official Full Name: transcriptional activator Myb isoform 3
• NCBI Official Synonym Full Names: v-myb avian myeloblastosis viral oncogene homolog
• NCBI Official Symbol: MYB
• NCBI Official Synonym Symbols: efg; Cmyb; c-myb; c-myb_CDS
• NCBI Protein Information: transcriptional activator Myb; c-myb8B_CDS; c-myb10A_CDS; c-myb13A_CDS; c-myb14A_CDS; proto-oncogene c-Myb; c-myb protein (140 AA)
• UniProt Protein Name: Transcriptional activator Myb
• Protein Family: Myb protein
• UniProt Gene Name: MYB
• UniProt Entry Name: MYB_HUMAN

NCBI Description

This gene encodes a transcription factor that is a member of the MYB family of transcription factor genes. The protein contains three domains, an N-terminal DNA-binding domain, a central transcriptional activation domain and a C-terminal domain involved in transcriptional repression. This protein plays an essential role in the regulation of hematopoiesis and may play a role in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2009]

Uniprot Description

Myb: Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 6q22-q23

Cellular Component: nuclear matrix; cytosol

Molecular Function: protein binding; chromatin binding

Biological Process: positive regulation of histone H3-K9 methylation; thymus development; transcription, DNA-dependent; in utero embryonic development; positive regulation of transcription, DNA-dependent; homeostasis of number of cells; negative regulation of transcription from RNA polymerase II promoter; positive regulation of histone H3-K4 methylation; chromatin remodeling; positive regulation of T-helper cell differentiation; regulation of transcription, DNA-dependent; calcium ion transport; B cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; blood coagulation; embryonic gut development; G1/S transition of mitotic cell cycle