Mouse anti-Human N myc (and STAT) Interactor/NMI Monoclonal Antibody | anti-NMI antibody

N myc (and STAT) Interactor/NMI Mouse Monoclonal Antibody [Clone PCRP-NMI-1C1]

Cat. Num. AAA4382354

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: N myc (and STAT) Interactor/NMI; Monoclonal Antibody; N myc (and STAT) Interactor/NMI Mouse Monoclonal Antibody [Clone PCRP-NMI-1C1]; N myc (and STAT) interactor; N myc interactor; NMI; NMYC and STAT interactor; NMYC interactor; anti-NMI antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse/IgG2b
• Clone Number: PCRP-NMI-1C1
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml; OR; Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)

• Applicable Applications for anti-NMI antibody: Flow Cytometry (FC/FACS)
• Application Notes: FC/FACS: 1-2ug/million cells
• Immunogen: Recombinant full-length human NMI protein
• Cellular Localization: Nucleus
• Positive Control: HeLa or K562 cells.
• Hu-chromosome Location: 2q23.3
• Unigene: 54483
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C; Antibody without azide: store at -20 to -80 degree C; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Flow Cytometry (FC/FACS)

(Flow Cytometric Analysis of PFA-fixed HeLa cells. NMI Mouse Monoclonal Antibody (PCRP-NMI-1C1) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red). )

Related Product Information for anti-NMI antibody

Nmi (for N-Myc interactor) is an interferon inducible protein that associates with multiple transcription factors, including c-Myc, n-Myc, Max, and c-Fos, which contain bHLH-ZIP, bHLH, or Zip domains. Nmi is ubiquitously expressed at low levels throughout various fetal and adult tissues and at higher levels in myeloid leukemias and cell lines overexpressing c-Myc. In addition to binding Myc proteins, Nmi also associates with the Stat family of transcription factors, where it enhances Stat-dependent transcription. Although Nmi lacks an intrinsic DNA binding or activation domain, Nmi enhances the transcriptional activity of the Stat proteins, in response to cytokine stimulation, by recruiting the Stat1 and Stat5 transcriptional coactivators, CREB-binding protein (CBP) and p300. In vitro studies indicate that Nmi, expressed in conjunction with CBP, enhances the transcriptional responsiveness of Stat5 to IL-2 stimulation five-fold over CBP alone by increasing the affinity of Stat proteins for CBP/p300.

NCBI and Uniprot Product Information

• NCBI GI #: 188219599
• NCBI GeneID: 9111
• NCBI Accession #: NP_004679.2
• NCBI GenBank Nucleotide #: NM_004688.2
• UniProt Accession #: Q13287; Q53TI8; Q9BVE5; B5BU69
• Molecular Weight: 35,057 Da
• NCBI Official Full Name: N-myc-interactor
• NCBI Official Synonym Full Names: N-myc (and STAT) interactor
• NCBI Official Symbol: NMI
• NCBI Protein Information: N-myc-interactor; N-myc interactor
• UniProt Protein Name: N-myc-interactor
• Protein Family: N-myc-interactor
• UniProt Gene Name: NMI
• UniProt Synonym Gene Names: Nmi
• UniProt Entry Name: NMI_HUMAN

NCBI Description

NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias. [provided by RefSeq, Jul 2008]

Uniprot Description

NMI: May be involved in augmenting coactivator protein recruitment to a group of sequence-specific transcription factors. Augments cytokine-mediated STAT transcription. Enhances CBP/p300 coactivator protein recruitment to STAT1 and STAT5. Belongs to the NMI family.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 2q23

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein binding; transcription cofactor activity

Biological Process: transcription from RNA polymerase II promoter; inflammatory response; JAK-STAT cascade