Mouse GRAMD4 Monoclonal Antibody | anti-GRAMD4 antibody

GRAMD4 (Transcription Factor) Mouse Monoclonal Antibody [Clone PCRP-GRAMD4-1A10]

Cat. Num. AAA4381897

• Gene Names: GRAMD4; DIP; dA59H18.1; dJ439F8.1
• Reactivity: Human; Predicted to react in Rat.
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: GRAMD4; Monoclonal Antibody; GRAMD4 (Transcription Factor) Mouse Monoclonal Antibody [Clone PCRP-GRAMD4-1A10]; GRAMD4 (Transcription Factor); Death-inducing protein; GRAM domain-containing protein 4 (GRAMD4); anti-GRAMD4 antibody

• Host: Mouse
• Reactivity: Human; Predicted to react in Rat.
• Clonality: Monoclonal
• Isotype: Mouse/IgG1
• Clone Number: PCRP-GRAMD4-1A10
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml; OR; Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)

• Applicable Applications for anti-GRAMD4 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: FC/FACS: 1-2ug/million cells; IF: 1-2ug/ml
• Immunogen: Recombinant full-length human GRAMD4 protein
• Cellular Localization: Mitochondrion membrane, Colocalizes with COX4I1
• Positive Control: Human liver, heart or fetal brain.
• Hu-chromosome Location: 22q13.31
• Unigene: 475150
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C; Antibody without azide: store at -20 to -80 degree C; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Flow Cytometry (FC/FACS)

(Flow Cytometric Analysis of PFA-fixed HeLa cells. GRAMD4 Mouse Monoclonal Antibody (PCRP-GRAMD4-1A10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red). )

Related Product Information for anti-GRAMD4 antibody

The gene encoding GRAMD4 (GRAM domain-containing protein 4) maps to human chromosome 22, which houses over 500 genes and is the second smallest human chromosome. GRAMD4, also designated death-inducing protein (DIP), is a 578 amino acid mitochondrial membrane protein that acts as an essential mediator of the p53-independent E2F-1 death pathway, which is frequently found to be deregulated in several types of cancers. Overexpression of GRAMD4 results in a strong apoptotic response involving caspase-3 activation and cleavage of poly(ADP-ribose)-polymerase. GRAMD4 is expressed in lung and in primary lung squamous cell carcinoma (LSCC) and shows upregulation in mitochondria by E2F1 after addition of 4-hydroxytamoxifen. This evidence suggests that GRAMD4 may be a potential target for cancer therapies.

NCBI and Uniprot Product Information

• NCBI GI #: 67763814
• NCBI GeneID: 23151
• NCBI Accession #: NP_055939.1
• NCBI GenBank Nucleotide #: NM_015124.4
• UniProt Accession #: Q6IC98; Q68EN0; Q9UGE6; Q9Y4B9; A9IN51; A9IN57
• Molecular Weight: 10,923 Da
• NCBI Official Full Name: GRAM domain-containing protein 4
• NCBI Official Synonym Full Names: GRAM domain containing 4
• NCBI Official Symbol: GRAMD4
• NCBI Official Synonym Symbols: DIP; dA59H18.1; dJ439F8.1
• NCBI Protein Information: GRAM domain-containing protein 4
• UniProt Protein Name: GRAM domain-containing protein 4
• Protein Family: GRAM domain-containing protein
• UniProt Gene Name: GRAMD4
• UniProt Synonym Gene Names: DIP; KIAA0767
• UniProt Entry Name: GRAM4_HUMAN

NCBI Description

GRAMD4 is a mitochondrial effector of E2F1 (MIM 189971)-induced apoptosis (Stanelle et al., 2005 [PubMed 15565177]).[supplied by OMIM, Jan 2011]