ELISA (EIA)
(Sandwich ELISA analysis of human tPA using Mouse anti Human tPA as a capture reagent and biotinylated Mouse anti Human tPA (MBS226243) as a detection reagent with purified native tPA as the antigen. Detection was performed using HRP conjugated )
Mouse anti-Human Tpa Monoclonal Antibody | anti-tPA antibody
Mouse anti Human Tpa:Biotin
Gene Names
PLAT; TPA; T-PA
Reactivity
Human
Applications
ELISA
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Synonyms
Tpa; Monoclonal Antibody; Mouse anti Human Tpa:Biotin; Tpa antibody | D12-9A7; anti-tPA antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
D12-9A7
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Form/Format
Phosphate buffered saline
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Purified IgG conjugated to Biotin - liquid
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Purified IgG conjugated to Biotin - liquid
Concentration
IgG concentration 0.1mg/ml (varies by lot)
Applicable Applications for anti-tPA antibody
ELISA (EIA)
Application Notes
ELISA: 1.0ug/ml
Immunogen
Purified native human tPA
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
Conjugation
Biotin
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
ELISA (EIA)
(Sandwich ELISA analysis of human tPA using Mouse anti Human tPA as a capture reagent and biotinylated Mouse anti Human tPA (MBS226243) as a detection reagent with purified native tPA as the antigen. Detection was performed using HRP conjugated )
ELISA (EIA)
(Sandwich ELISA analysis of human tPA using Mouse anti Human tPA as a capture reagent and biotinylated Mouse anti Human tPA (MBS226243) as a detection reagent with purified native tPA as the antigen. Detection was performed using HRP conjugated )
Related Product Information for anti-tPA antibody
Mouse anti Human tPA antibody, clone D12-9A7, recognizes tissue type plasminogen activator (tPA) also known as PLAT. tPA is a serine protease that in the intravascular space, catalyzes the conversion of plasminogen to plasmin. tPA is expressed in various cell types including endothelial cells, glial cells and neurons. Within the brain tPA plays a role in synaptic plasticity, detection and adaptation to metabolic stress, modulation of the blood-brain barrier permeability and remodeling of the extracellular matrix (Jeanneret & Yepes 2017).
The biotinylated Mouse anti Human tPA antibody, clone D12-9A7 can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human tPA antibody, clone D11-7G8 as the capture antibody.
The biotinylated Mouse anti Human tPA antibody, clone D12-9A7 can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human tPA antibody, clone D11-7G8 as the capture antibody.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
62,917 Da
NCBI Official Full Name
tissue-type plasminogen activator
NCBI Official Synonym Full Names
plasminogen activator, tissue
NCBI Official Symbol
PLAT
NCBI Official Synonym Symbols
TPA; T-PA
NCBI Protein Information
tissue-type plasminogen activator; alteplase; reteplase; t-plasminogen activator; plasminogen activator, tissue type; tissue plasminogen activator (t-PA)
UniProt Protein Name
Tissue-type plasminogen activator
Protein Family
UniProt Gene Name
PLAT
UniProt Synonym Gene Names
t-PA; t-plasminogen activator; tPA
UniProt Entry Name
TPA_HUMAN
Similar Products
Product Notes
The tPA plat (Catalog #AAA226243) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Human Tpa:Biotin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Tpa can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). ELISA: 1.0ug/ml. Researchers should empirically determine the suitability of the tPA plat for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tpa, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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