Mouse Golgi Complex Monoclonal Antibody
Golgi Complex (Marker for Human Cells)
Reactivity
HumanDoes not react with Mouse or Rat.
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
Purity
Purified Ab conjugated to CF700
Synonyms
Golgi Complex; Monoclonal Antibody; Golgi Complex (Marker for Human Cells); Golgi apparatus; Golgi zone; anti-Golgi Complex antibody
Host
Mouse
Reactivity
Human
Does not react with Mouse or Rat.
Does not react with Mouse or Rat.
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
371-4
Specificity
This MAb recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae. The Golgi tends to be more pronounced and numerous in cells that make and secrete many substances such as plasma B cells.
Purity/Purification
Purified Ab conjugated to CF700
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Applicable Applications for anti-Golgi Complex antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
Application Notes
FC/FACS: 0.5-1.0ug/million cells
IF: 0.5-1ug/ml
WB: 0.5-1.0ug/ml
IF: 0.5-1ug/ml
WB: 0.5-1.0ug/ml
Positive Control
HepG2, A431 or HeLa cells. Tonsil or lymph node.
Immunogen
SU-DHL-1 large cell lymphoma cells.
Cellular Localization
Golgi complex in cytoplasm
Preparation and Storage
Antibody with azide: store at 4 to 8 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
References
1. Yuasa, K et. al. Binding and Phosphorylation of a Novel Male Germ Cell-specific cGMP-dependent Protein Kinase-anchoring Protein by cGMP-dependent Protein Kinase I. J Biol Chem, 275(7):4897-4905;2000. 2. Yoshio Endo et. al. Cellular localization and functional characterization of the equilibrative nucleoside transporters of antitumor nucleosides. Cancer science 98;2007
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Product Notes
The Golgi Complex (Catalog #AAA4381461) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Golgi Complex (Marker for Human Cells) reacts with Human Does not react with Mouse or Rat. and may cross-react with other species as described in the data sheet. AAA Biotech's Golgi Complex can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB). FC/FACS: 0.5-1.0ug/million cells IF: 0.5-1ug/ml WB: 0.5-1.0ug/ml. Researchers should empirically determine the suitability of the Golgi Complex for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Golgi Complex, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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