Mouse anti-Human CD71/Transferrin Receptor (TFRC) Monoclonal Antibody | anti-TFRC antibody

CD71/Transferrin Receptor (TFRC)

Cat. Num. AAA4381284

• Gene Names: TFRC; T9; TR; TFR; p90; CD71; TFR1; TRFR; IMD46
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Purified Ab conjugated to CF488
• Synonyms: CD71/Transferrin Receptor (TFRC); Monoclonal Antibody; Mtvr-1; p90; TFR1; TFRC transferrin receptor (p90 CD71); TRFR; anti-TFRC antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: 66IG10
• Specificity: It recognizes a ~90-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa. This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Its epitope is localized in the extracellular domain of CD71. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.
• Purity/Purification: Purified Ab conjugated to CF488
• Form/Format: Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.
• Sequence Length: 760

• Applicable Applications for anti-TFRC antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: FC/FACS: 5ul per test per one million cells or 5ul per 100ul of whole blood; IF: 1:50-1:100
• Positive Control: CCRF-CEM, Jurkat or K-562. Human placenta or breast carcinoma.
• Immunogen: Human thymocytes (T-cells)
• Cellular Localization: Cell Surface
• Hu-chromosome Location: 3q29
• Preparation and Storage: Antibody with azide: store at 4 to 8 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Flow Cytometry (FC/FACS)

(Flow Cytometry of human CD71 on K562 cells. Black: cells alone; Grey: Isotype Control; Green: CF488-labeled CD71 Monoclonal Antibody (66IG10).)

References

1. K. Moolenaar et al. Cancer research 50,1102-1106, 1990. 2. Van de Rijna M, Geurts van Kessel AHM, Kroezen V, van Agthoven AJ, Verstijnen K, Terhorst C, Hilgers J: Cytogenet Cell Genet 1983;36:525-531. 3. Oudermans et al. Cancer, 1986; 58:1252

NCBI and Uniprot Product Information

• NCBI GI #: 189458819
• NCBI GeneID: 7037
• NCBI Accession #: NP_001121620.1
• NCBI GenBank Nucleotide #: NP_001121620.1
• UniProt Accession #: P02786
• Molecular Weight: 85-95kDa (monomer); 190kDa (dimer)
• NCBI Official Full Name: transferrin receptor protein 1 isoform 1
• NCBI Official Synonym Full Names: transferrin receptor
• NCBI Official Symbol: TFRC
• NCBI Official Synonym Symbols: T9; TR; TFR; p90; CD71; TFR1; TRFR; IMD46
• NCBI Protein Information: transferrin receptor protein 1
• UniProt Protein Name: Transferrin receptor protein 1
• Protein Family: Transferrin receptor protein
• UniProt Gene Name: TFRC
• UniProt Synonym Gene Names: TR; TfR; TfR1; Trfr; sTfR
• UniProt Entry Name: TFR1_HUMAN

NCBI Description

This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]

Uniprot Description

TfR: the transferrin receptor. Regulates the cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied receptors into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system.

Protein type: Membrane protein, integral; Receptor, misc.; Cell surface

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; integral to plasma membrane; cytoplasmic membrane-bound vesicle; extracellular region; coated pit; recycling endosome; membrane; perinuclear region of cytoplasm; melanosome; plasma membrane; vesicle; endosome; external side of plasma membrane

Molecular Function: identical protein binding; protein binding; transferrin receptor activity; double-stranded RNA binding

Biological Process: receptor-mediated endocytosis; viral reproduction; cellular iron ion homeostasis; positive regulation of bone resorption; transferrin transport; osteoclast differentiation; regulation of cell growth; transmembrane transport; regulation of cell proliferation