Rabbit anti-Human MEKK3 Polyclonal Antibody | anti-MEKK3 antibody

MEKK3 (MAPK/ERK Kinase Kinase 3, MEK Kinase 3, MEKK 3, Mitogen-activated Protein Kinase Kinase Kinase 3, MAP3K3, MAPKKK3) (FITC)

Cat. Num. AAA6385272

• Gene Names: MAP3K3; MEKK3; MAPKKK3
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MEKK3; Polyclonal Antibody; MEKK3 (MAPK/ERK Kinase Kinase 3; MEK Kinase 3; MEKK 3; Mitogen-activated Protein Kinase Kinase Kinase 3; MAP3K3; MAPKKK3) (FITC); anti-MEKK3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MAP3K3.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 2067

• Applicable Applications for anti-MEKK3 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human MAP3K3, aa1-90 (AAH10464.1).
• Immunogen Sequence: MNEANVMLPYSGKEEPVLPVAMTLPLPGRGPRCGTAATEGGSSFVNAVVSVLQVGVTLMLYPVSKLETVCALWALSTPALGLGLGCIEKS
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MAP3K3 rabbit polyclonal antibody. Western Blot analysis of MAP3K3 expression in HeLa.)

Testing Data

(Proximity Ligation Analysis of protein-protein interactions between MAP3K3 and MAP2K5 HeLa cells were stained with anti-MAP3K3 rabbit purified polyclonal 1:1200 and anti-MAP2K5 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Related Product Information for anti-MEKK3 antibody

MEKK3 directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhances transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway.

Product Categories/Family for anti-MEKK3 antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 14714648
• NCBI GeneID: 4215
• NCBI Official Full Name: Homo sapiens mitogen-activated protein kinase kinase kinase 3, mRNA
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 3
• NCBI Official Symbol: MAP3K3
• NCBI Official Synonym Symbols: MEKK3; MAPKKK3
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 3

NCBI Description

This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]