Rabbit anti-Human Ep-CAM/CD326 Antibody | anti-Ep-CAM/CD326 antibody

Ep-CAM/CD326 (Extracellular Domain) (Epithelial Marker)

Cat. Num. AAA4381073

• Gene Names: EPCAM; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Ep-CAM/CD326; Ep-CAM/CD326 (Extracellular Domain) (Epithelial Marker); Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TROP1; Tumor-associated Calcium Signal Transducer 1 (TACSTD1); anti-Ep-CAM/CD326 antibody

• Host: Rabbit
• Reactivity: Human
• Isotype: IgG
• Clone Number: EGP40/2571R
• Specificity: Epitope of this MAb is mapped between aa 202-212 of EGP40, which is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 314

• Applicable Applications for anti-Ep-CAM/CD326 antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Paraffin
• Application Notes: FC/FACS: 1-2ug/million cells; Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: MCF-7 or HT29 cells. Breast tumor.
• Immunogen: Recombinant human EpCAM fragment from extracellular domain (around aa100-224) (exact sequence is proprietary)
• Cellular Localization: Cell Surface
• Hu-chromosome Location: 2q21
• Antibody Type: Recombinant Rabbit Monoclonal Antibody
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with EpCAM Rabbit Recombinant Monoclonal Antibody (EGP40/2571R).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with EpCAM Rabbit Recombinant Monoclonal Antibody (EGP40/2571R).)

SDS-Page

(SDS-PAGE Analysis Purified EpCAM Rabbit Recombinant Monoclonal Antibody (EGP40/2571R). Confirmation of Integrity and Purity of Antibody.)

Flow Cytometry (FC/FACS)

(Flow Cytometric Analysis of MCF-7 cells using EpCAM Rabbit Recombinant Monoclonal Antibody (EGP40/2571R) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)

References

Bjork, P., et al. 1993. J. Biol. Chem. 268: 24232-24241.

NCBI and Uniprot Product Information

• NCBI GI #: 218505670
• NCBI GeneID: 4072
• NCBI Accession #: NP_002345.2
• NCBI GenBank Nucleotide #: NP_002345.2
• UniProt Accession #: P16422
• Molecular Weight: 40-43kDa
• NCBI Official Full Name: epithelial cell adhesion molecule
• NCBI Official Synonym Full Names: epithelial cell adhesion molecule
• NCBI Official Symbol: EPCAM
• NCBI Official Synonym Symbols: ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
• NCBI Protein Information: epithelial cell adhesion molecule
• UniProt Protein Name: Epithelial cell adhesion molecule
• UniProt Gene Name: EPCAM
• UniProt Synonym Gene Names: GA733-2; M1S2; M4S1; MIC18; TACSTD1; TROP1; Ep-CAM; EGP; EGP314; hEGP314
• UniProt Entry Name: EPCAM_HUMAN

NCBI Description

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

Uniprot Description

EPCAM: a single-pass type I membrane protein of the EPCAM family. May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinomas including pancreatic carcinoma. May be a marker of cancer stem cells.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p21

Cellular Component: tight junction; cell surface; basolateral plasma membrane; apical plasma membrane; integral to membrane; plasma membrane; lateral plasma membrane

Molecular Function: protein binding; protein complex binding

Biological Process: ureteric bud development; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; stem cell differentiation

Disease: Diarrhea 5, With Tufting Enteropathy, Congenital; Colorectal Cancer, Hereditary Nonpolyposis, Type 8