Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-human IgG Rabbit Polyclonal Antibody.)
Rabbit IgG (Immunoglobulin Gamma Heavy Chain) Polyclonal Antibody | anti-IGHG antibody
IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker)
Reactivity
Human. Others not known.
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
Synonyms
IgG (Immunoglobulin Gamma Heavy Chain); Polyclonal Antibody; IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker); G1m Marker; G2m Marker; G3m Marker; G4m Marker; HDC; Heavy Chain Disease Protein; Human Immunglobulin G; Ig gamma1/2/3/4 Chain C Region; IGHG1; IGHG2; IGHG3; IGHG4; Immunoglobulin Heavy Constant ¯§ 1/2/3/4; anti-IGHG antibody
Host
Rabbit
Reactivity
Human. Others not known.
Clonality
Polyclonal
Isotype
IgG, kappa
Specificity
Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Form/Format
200ug/ml of Ab purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
330
Applicable Applications for anti-IGHG antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes
FC/FACS: 0.5-1ug/million cells
IF: 0.5-1ug/ml
IHC-F: 0.5-1ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
IF: 0.5-1ug/ml
IHC-F: 0.5-1ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Immunogen
Purified human Ig Gamma Chain
Cellular Localization
Cytoplasm, Cell Surface and Secreted
Hu-chromosome Location
14q32.33
Positive Control
293T, Raji or hPBL cells. Tonsil or Spleen.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-human IgG Rabbit Polyclonal Antibody.)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-human IgG Rabbit Polyclonal Antibody.)
References
Horejsi, V., et al. 1986. Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens. Tissue Antigens 28: 288-297. Brodsky FM. A matrix approach to human class II histocompatibility antigens: reactions of four monoclonal antibodies with the products of nine haplotypes. Immunogenetics. 1984; 19(3):179-94. Engleman EG, Warnke R, Fox RI, Dilley J, Benike CJ, Levy R. Studies of a human T lymphocyte antigen recognized by a monoclonal antibody. Proc Natl Acad Sci U S A. 1981; 78(3):1791-5.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
75kDa
NCBI Official Full Name
Immunoglobulin heavy constant gamma 1
NCBI Official Synonym Full Names
immunoglobulin heavy constant gamma 1 (G1m marker)
NCBI Official Symbol
IGHG1
UniProt Protein Name
Immunoglobulin heavy constant gamma 1
Protein Family
UniProt Gene Name
IGHG1
Uniprot Description
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).
Research Articles on IGHG
1. Our results show that immunoglobulin GM genes are risk factors for the development of schizophrenia
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Product Notes
The IGHG ighg1 (Catalog #AAA4380033) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker) reacts with Human. Others not known. and may cross-react with other species as described in the data sheet. AAA Biotech's IgG (Immunoglobulin Gamma Heavy Chain) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin. FC/FACS: 0.5-1ug/million cells IF: 0.5-1ug/ml IHC-F: 0.5-1ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Researchers should empirically determine the suitability of the IGHG ighg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG (Immunoglobulin Gamma Heavy Chain), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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