Interleukin 4 Active Protein | IL-4 active protein

Human Interleukin 4 (IL-4), yeast

Cat. Num. AAA434178

• Gene Names: IL4; BSF1; IL-4; BCGF1; BSF-1; BCGF-1
• Purity: > or = 97% purity (by SDS-PAGE and HPLC)
• Synonyms: Interleukin 4; Human Interleukin 4 (IL-4); yeast; human interleukin 4; IL-4 active protein

• Purity/Purification: > or = 97% purity (by SDS-PAGE and HPLC)
• Form/Format: Lyophilized from a 0.2 um filtered solution in PBS
• Sequence Length: 153

• Source: Yeast
• Endotoxin Level: <=1 EU/mg, determined by the LAL method
• Biological Activity: Measured in a cell proliferation assay using a human factor-dependent cell line, TF-1. The specific activity shall be not less than 1×10⁷IU/mg.
• Reconstitution: Reconstitute with double distilled water at a concentration of no less than 100 ug/ml with 0.1% human serum albumin (A highly purified plant-derived human serum albumin is strongly suggested to be used) or Bovine serum albumin as a stock.
• Preparation and Storage: Stable for 6-months from the date of shipment when kept at -20 degree C or -70 degree C. Upon reconstitution, it can be stored at 4 degree C for at least one month or -20 degree C for at least three months. Avoid repeated freeze-thaw cycles.

Related Product Information for IL-4 active protein

Description: Recombinant human IL-4 produced in Yeast is a single, glycosylated, polypeptide chain containing 129 amino acids, three pairs of disulfide bonds and having a molecular mass of approximately 15.0kD (after glycosylation, the molecular mass is 30.0kD).
Introduction: Interleukin 4 is a pleiotropic cytokine produced by activated T cells, mast cells, and basophils. It was initially identified as a B cell differentiation factor (BCDF), as well as a B cell stimulatory factor (BSF1). Subsequent to the molecular cloning and expression of both human and Mouse IL-4, numerous other functions have been described on B cells as well as other hematopoietic and nonhematopoietic cells, including T lymphocytes, monocytes, macrophages, mast cells, myeloid and erythroid progenitors, fibroblasts, endothelial cells, etc. IL-4 exhibits anti-tumor effects both in vivo and in vitro.

Product Categories/Family for IL-4 active protein

Cytokine Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4504669
• NCBI GeneID: 3565
• NCBI Accession #: NP_000580.1
• NCBI GenBank Nucleotide #: NM_000589.3
• NCBI Official Full Name: interleukin-4 isoform 1
• NCBI Official Synonym Full Names: interleukin 4
• NCBI Official Symbol: IL4
• NCBI Official Synonym Symbols: BSF1; IL-4; BCGF1; BSF-1; BCGF-1
• NCBI Protein Information: interleukin-4; B cell growth factor 1; B_cell stimulatory factor 1; binetrakin; interleukin 4 variant 2; lymphocyte stimulatory factor 1; pitrakinra
• UniProt Protein Name: Interleukin-4
• UniProt Gene Name: IL4
• UniProt Entry Name: IL4_HUMAN

NCBI Description

The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

IL4: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the IL-4/IL-13 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Secreted, signal peptide; Cell cycle regulation; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; external side of plasma membrane

Molecular Function: protein binding; growth factor activity; cytokine activity; interleukin-4 receptor binding

Biological Process: regulation of isotype switching; negative regulation of osteoclast differentiation; positive regulation of isotype switching to IgG isotypes; positive regulation of transcription, DNA-dependent; regulation of proton transport; microglial cell activation; female pregnancy; chemotaxis; positive regulation of activated T cell proliferation; response to organic cyclic substance; positive regulation of isotype switching to IgE isotypes; positive regulation of interleukin-10 production; B cell costimulation; positive regulation of MHC class II biosynthetic process; connective tissue growth factor biosynthetic process; positive regulation of B cell proliferation; positive regulation of T cell proliferation; positive regulation of interleukin-13 production; negative regulation of macrophage activation; response to nutrient; response to drug; cholesterol metabolic process; regulation of immune response; negative regulation of chronic inflammatory response; T-helper 2 type immune response; B cell activation; regulation of phosphorylation; negative regulation of nitric oxide biosynthetic process; negative regulation of acute inflammatory response; defense response to protozoan; T-helper 1 cell lineage commitment; positive regulation of chemokine biosynthetic process; response to ethanol; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of T cell differentiation; innate immune response in mucosa; retina development in camera-type eye; response to cytokine stimulus; B cell differentiation; cellular defense response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; immune response; T-helper 2 cell differentiation; positive regulation of defense response to virus by host; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis

Disease: Stroke, Ischemic