Rat Apolipoprotein A5 ELISA Kit | APOA5 elisa kit

Rat Apolipoprotein A5 ELISA Kit

Cat. Num. AAA42486

• Gene Names: APOA5; RAP3; APOAV
• Reactivity: Rat
• Synonyms: Apolipoprotein A5; Rat Apolipoprotein A5 ELISA Kit; APOA5 elisa kit

• Reactivity: Rat
• Specificity: No significant cross-reactivity or interference between Rat APOA5 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 31.2ng/ml-1000ng/ml.
• Sensitivity: 5.0ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated APOA5 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat APOA5 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add APOA5 and APOA5 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of APOA5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for APOA5 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated APOA5 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat APOA5 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add APOA5 and APOA5 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of APOA5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 262231737
• NCBI GeneID: 116519
• NCBI Accession #: NP_001160070.1
• NCBI GenBank Nucleotide #: NM_001166598.1
• UniProt Accession #: Q6Q788; Q3MIK6; Q6UWK9; Q9UBJ3; B0YIV9
• Molecular Weight: 41,213 Da
• NCBI Official Full Name: apolipoprotein A-V
• NCBI Official Synonym Full Names: apolipoprotein A-V
• NCBI Official Symbol: APOA5
• NCBI Official Synonym Symbols: RAP3; APOAV
• NCBI Protein Information: apolipoprotein A-V; apo-AV; apolipoprotein A5; regeneration-associated protein 3
• UniProt Protein Name: Apolipoprotein A-V
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOA5
• UniProt Synonym Gene Names: RAP3; Apo-AV; ApoA-V
• UniProt Entry Name: APOA5_HUMAN

NCBI Description

The protein encoded by this gene is an apolipoprotein that plays an important role in regulating the plasma triglyceride levels, a major risk factor for coronary artery disease. It is a component of high density lipoprotein and is highly similar to a rat protein that is upregulated in response to liver injury. Mutations in this gene have been associated with hypertriglyceridemia and hyperlipoproteinemia type 5. This gene is located proximal to the apolipoprotein gene cluster on chromosome 11q23. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Oct 2009]

Uniprot Description

APOA5: Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and a inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate). Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages. Interacts with GPIHBP1. Up-regulated by PPARA agonists, which are used clinically to lower serum TG (such as fibrates). Liver and plasma. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Secreted, signal peptide; Secreted; Lipid-binding

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: extracellular space; chylomicron; extracellular region

Molecular Function: heparin binding; enzyme binding; low-density lipoprotein receptor binding; cholesterol transporter activity; enzyme activator activity; cholesterol binding; phospholipid binding; phosphatidylcholine binding; lipid binding

Biological Process: positive regulation of fatty acid biosynthetic process; organ regeneration; response to hormone stimulus; neurite regeneration; lipoprotein metabolic process; cholesterol efflux; positive regulation of lipid catabolic process; cellular lipid metabolic process; lipid transport; cholesterol biosynthetic process; positive regulation of receptor-mediated endocytosis; phosphatidylcholine metabolic process; cholesterol homeostasis; reverse cholesterol transport; triacylglycerol metabolic process; phospholipid efflux; regulation of cholesterol absorption; acylglycerol homeostasis; tissue regeneration; triacylglycerol catabolic process; positive regulation of lipoprotein lipase activity

Disease: Hypertriglyceridemia, Familial; Hyperlipoproteinemia, Type V