Cystatin A / CSTA Recombinant Protein | CSTA recombinant protein

Recombinant Human Cystatin A / CSTA Protein (His tag)

Cat. Num. AAA2546234

• Gene Names: CSTA; AREI; PSS4; STF1; STFA
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: Cystatin A / CSTA; Recombinant Human Cystatin A / CSTA Protein (His tag); STF1; STFA; CSTA recombinant protein

• Host: E Coli
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 1mM EDTA, pH 7.5
• Sequence Length: 98

• Application Notes: The recombinant human CSTA consisting of 108 amino acids and has a calculated molecular mass of 12.4 KDa. It migrates as an approximately 14 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CSTA recombinant protein

Background: Cystatin-A, also known as Cystatin-AS, Stefin-A and CSTA, is a cytoplasm protein which belongs to the cystatin family. Cystatin-A / CSTA is a cysteine proteinase inhibitor with a molecular mass of 11 kDa, and is located mainly in the keratohyaline granules of the stratum granulosum and the cornified envelope of the stratum corneum in the epidermis. The cystatins are a family of cysteine protease inhibitors with homology to chicken cystatin. Cystatins are physiological inhibitors of cysteine proteinases which are widely distributed in human tissues and fluids. Cystatins typically comprise about 115 amino acids, are largely acidic, contain four conserved cysteine residues known to form two disulfide bonds. Cystatins may be glycosylated and / or phosphorylated, with similarity to fetuins, kininogens, stefins, histidine-rich glycoproteins and cystatin-related proteins. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired inhibitory activity. Cystatins mainly inhibit peptidases belonging to peptidase families C1 (papain family) and C13 (legumain family).

Description: A DNA sequence encoding the human CSTA (P01040) (Ile 2-Phe 98) was expressed with a polyhistide tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4885165
• NCBI GeneID: 1475
• NCBI Accession #: NP_005204.1
• NCBI GenBank Nucleotide #: NM_005213.3
• UniProt Accession #: P01040; Q6IB90
• Molecular Weight: 11,006 Da
• NCBI Official Full Name: cystatin-A
• NCBI Official Synonym Full Names: cystatin A
• NCBI Official Symbol: CSTA
• NCBI Official Synonym Symbols: AREI; PSS4; STF1; STFA
• NCBI Protein Information: cystatin-A
• UniProt Protein Name: Cystatin-A
• Protein Family: Cystatin
• UniProt Gene Name: CSTA
• UniProt Synonym Gene Names: STF1; STFA

NCBI Description

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

CSTA: This is an intracellular thiol proteinase inhibitor. Has an important role in desmosome-mediated cell-cell adhesion in the lower levels of the epidermis. Defects in CSTA are the cause of ichthyosis exfoliative autosomal recessive ichthyosis bullosa of Siemens-like (AREI). A form of congenital exfoliative ichthyosis, sharing some features with ichthyosis bullosa of Siemens and annular epidermolytic ichthyosis. AREI presents shortly after birth as dry, scaly skin over most of the body with coarse peeling of non- erythematous skin on the palms and soles, which is exacerbated by excessive moisture and minor trauma. Electron microscopy analysis of skin biopsies, reveals mostly normal-appearing upper layers of the epidermis, but prominent intercellular edema of the basal and suprabasal cell layers with aggregates of tonofilaments in the basal keratinocytes. Belongs to the cystatin family.

Chromosomal Location of Human Ortholog: 3q21.1

Cellular Component: cornified envelope; cytoplasm; extracellular matrix; extracellular space; nucleus

Molecular Function: cysteine protease inhibitor activity; protease binding; protein binding, bridging; structural molecule activity

Biological Process: cell-cell adhesion; keratinocyte differentiation; negative regulation of peptidase activity; negative regulation of proteolysis; peptide cross-linking

Disease: Exfoliative Ichthyosis, Autosomal Recessive, Ichthyosis Bullosa Of Siemens-like