ALDH5A1 Antibody | anti-ALDH5A1 antibody

Goat anti-ALDH5A1 (aa 485-496), Biotinylated Antibody

Cat. Num. AAA423746

• Gene Names: ALDH5A1; SSDH; SSADH
• Reactivity: Human; Expected from sequence similarity: Mouse, Rat, Dog, Cow
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: ALDH5A1; Goat anti-ALDH5A1 (aa 485-496); Biotinylated Antibody; Goat Anti-ALDH5A1 (aa 485-496) Antibody; aldehyde dehydrogenase 5 family; member A1; SSADH; SSDH; NAD(+)-dependent succinic semialdehyde dehydrogenase; aldehyde dehydrogenase 5A1; mitochondrial succinate semialdehyde dehydrogenase; succinate-semialdehyde dehydrogenase; anti-ALDH5A1 antibody

• Reactivity: Human; Expected from sequence similarity: Mouse, Rat, Dog, Cow
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Sequence Length: 548

• Applicable Applications for anti-ALDH5A1 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Pep-ELISA: antibody detection limit dilution 1:32000.; WB: Approx 55kDa band observed in Human Liver lysates. Recommended concentration: 0.3-1ug/ml.
• Immunogen Sequence: Peptide with sequence C-SQDPAQIWRVAE., from the internal region of the protein sequence according to NP_733936.1; NP_001071.1.
• Peptide Sequence + C: C-SQDPAQIWRVAE.
• Epitope: Internal Region
• Note: This antibody is expected to recognize both reported isoforms (NP_733936.1; NP_001071.1). Amino acid numbering in name calculated from NP_733936.1.
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Biotinylated (0.5ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)

NCBI and Uniprot Product Information

• NCBI GI #: 25777721
• NCBI GeneID: 7915
• NCBI Accession #: NP_733936.1; NP_001071.1
• NCBI GenBank Nucleotide #: NM_170740.1
• UniProt Accession #: P51649; Q546H9; Q8N3W6; B2RD26; G5E949
• Molecular Weight: 58.7kDa
• NCBI Official Full Name: succinate-semialdehyde dehydrogenase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: aldehyde dehydrogenase 5 family member A1
• NCBI Official Symbol: ALDH5A1
• NCBI Official Synonym Symbols: SSDH; SSADH
• NCBI Protein Information: succinate-semialdehyde dehydrogenase, mitochondrial
• UniProt Protein Name: Succinate-semialdehyde dehydrogenase, mitochondrial
• UniProt Gene Name: ALDH5A1
• UniProt Synonym Gene Names: SSADH

NCBI Description

This protein belongs to the aldehyde dehydrogenase family of proteins. This gene encodes a mitochondrial NAD(+)-dependent succinic semialdehyde dehydrogenase. A deficiency of this enzyme, known as 4-hydroxybutyricaciduria, is a rare inborn error in the metabolism of the neurotransmitter 4-aminobutyric acid (GABA). In response to the defect, physiologic fluids from patients accumulate GHB, a compound with numerous neuromodulatory properties. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes one step in the degradation of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA).