Western Blot (WB)
(Biotinylated (0. 01ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Goat TXNDC1/TMX Polyclonal Antibody | anti-TMX1 antibody
Goat anti-TXNDC1/TMX, biotinylated Antibody
Gene Names
TMX1; TMX; TXNDC; PDIA11; TXNDC1
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
TXNDC1/TMX; Polyclonal Antibody; Goat anti-TXNDC1/TMX; biotinylated Antibody; Goat Anti-TXNDC1 / TMX Antibody; TMX1; thioredoxin-related transmembrane protein 1; PDIA11; TMX; TXNDC; TXNDC1; protein disulfide isomerase family A; member 11; thioredoxin domain containing 1; thioredoxin domain-containing protein 1; transmembrane Trx-related protein; TMX1 antibody; thioredoxin-related transmembrane protein 1 antibody; PDIA11 antibody; TMX antibody; TXNDC antibody; TXNDC1 antibody; member 11 antibody; thioredoxin domain containing 1 antibody; thioredoxin domain-containing protein 1 antibody; transmembrane Trx-related protein antibody; TXNDC1 / TMX; biotinylated; anti-TMX1 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
RSLGPSLATDKS
Sequence Length
280
Applicable Applications for anti-TMX1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 35kDa band observed in Human Liver lysates (calculated MW of 31.8kDa according to NP_110382.3). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.01-0.03ug/ml.
Western Blot: Approx 35kDa band observed in Human Liver lysates (calculated MW of 31.8kDa according to NP_110382.3). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.01-0.03ug/ml.
Immunogen
Peptide with sequence C-RSLGPSLATDKS. , from the C Terminus of the protein sequence according to NP_110382.3.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
(Biotinylated (0. 01ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Western Blot (WB)
(Biotinylated (0. 01ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,791 Da
NCBI Official Full Name
thioredoxin-related transmembrane protein 1
NCBI Official Synonym Full Names
thioredoxin related transmembrane protein 1
NCBI Official Symbol
TMX1
NCBI Official Synonym Symbols
TMX; TXNDC; PDIA11; TXNDC1
NCBI Protein Information
thioredoxin-related transmembrane protein 1
UniProt Protein Name
Thioredoxin-related transmembrane protein 1
Protein Family
UniProt Gene Name
TMX1
UniProt Synonym Gene Names
TMX; TXNDC; TXNDC1
UniProt Entry Name
TMX1_HUMAN
NCBI Description
TXNDC1 is a thioredoxin (TXN; see MIM 187700)-related protein with disulfide reductase activity (Matsuo et al., 2001 [PubMed 11152479]).[supplied by OMIM, Mar 2008]
Uniprot Description
TXNDC1: May participate in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyze dithiol-disulfide exchange reactions.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 14q22.1
Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to membrane; nucleolus
Molecular Function: disulfide oxidoreductase activity; protein disulfide isomerase activity
Biological Process: cell redox homeostasis; protein folding
Research Articles on TMX1
Similar Products
Product Notes
The TMX1 tmx1 (Catalog #AAA423629) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-TXNDC1/TMX, biotinylated Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's TXNDC1/TMX can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx 35kDa band observed in Human Liver lysates (calculated MW of 31.8kDa according to NP_110382.3). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.01-0.03ug/ml. Researchers should empirically determine the suitability of the TMX1 tmx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RSLGPSLATD KS. It is sometimes possible for the material contained within the vial of "TXNDC1/TMX, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
