Western Blot (WB)
(Biotinylated (0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Goat Latexin Polyclonal Antibody | anti-LXN antibody
Goat anti-Latexin, Biotinylated Antibody
Gene Names
LXN; ECI; TCI
Reactivity
Tested: Human, Mouse, Rat, Dog; Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Latexin; Polyclonal Antibody; Goat anti-Latexin; Biotinylated Antibody; Goat Anti-Latexin Antibody; LXN; latexin; ECI; TCI; MUM; endogenous carboxypeptidase inhibitor; tissue carboxypeptidase inhibitor; LXN antibody; latexin antibody; ECI antibody; TCI antibody; MUM antibody; endogenous carboxypeptidase inhibitor antibody; tissue carboxypeptidase inhibitor antibody; Biotinylated; anti-LXN antibody
Host
Goat
Reactivity
Tested: Human, Mouse, Rat, Dog; Expected from sequence similarity: Human, Mouse, Rat, Dog
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
QYGTKVKHNSRLPKE
Sequence Length
222
Applicable Applications for anti-LXN antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 28kDa band observed in Rat Brain lysates (calculated MW of 25.6kDa according to Rat NP_113843.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3ug/ml.
Western Blot: Approx 28kDa band observed in Rat Brain lysates (calculated MW of 25.6kDa according to Rat NP_113843.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3ug/ml.
Immunogen
Peptide with sequence C-QYGTKVKHNSRLPKE. , from the C Terminus of the protein sequence according to NP_064554.3.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
(Biotinylated (0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Western Blot (WB)
(Biotinylated (0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
latexin
NCBI Official Synonym Full Names
latexin
NCBI Official Symbol
LXN
NCBI Official Synonym Symbols
ECI; TCI
NCBI Protein Information
latexin
UniProt Protein Name
Latexin
Protein Family
UniProt Gene Name
LXN
UniProt Synonym Gene Names
ECI; TCI
UniProt Entry Name
LXN_HUMAN
NCBI Description
This gene encodes the only known protein inhibitor of zinc-dependent metallocarboxypeptidases. [provided by RefSeq, Oct 2008]
Uniprot Description
LXN: Hardly reversible, non-competitive, and potent inhibitor of CPA1, CPA2 and CPA4. Belongs to the protease inhibitor I47 (latexin) family.
Chromosomal Location of Human Ortholog: 3q25.32
Cellular Component: cytoplasm
Molecular Function: heparin binding; metalloendopeptidase inhibitor activity; protein binding
Biological Process: detection of temperature stimulus involved in sensory perception of pain; inflammatory response
Research Articles on LXN
Similar Products
Product Notes
The LXN lxn (Catalog #AAA423581) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Latexin, Biotinylated Antibody reacts with Tested: Human, Mouse, Rat, Dog; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's Latexin can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 28kDa band observed in Rat Brain lysates (calculated MW of 25.6kDa according to Rat NP_113843.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the LXN lxn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QYGTKVKHNS RLPKE. It is sometimes possible for the material contained within the vial of "Latexin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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