Goat GEM Polyclonal Antibody | anti-GEM antibody

Goat anti-GEM (aa34-46) Antibody

Cat. Num. AAA423162

• Gene Names: GEM; KIR
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: GEM; Polyclonal Antibody; Goat anti-GEM (aa34-46) Antibody; Goat Anti-GEM / KIR Antibody; GTP binding protein overexpressed in skeletal muscle; KIR; MGC26294; GTP-binding mitogen-induced T-cell protein; GTP-binding protein GEM; GTP-binding protein overexpressed in skeletal muscle; OTTHUMP00000226600; OTTHUMP00000226601; RAS-like protein K; GEM antibody; GTP binding protein overexpressed in skeletal muscle antibody; GTP-binding mitogen-induced T-cell protein antibody; GTP-binding protein GEM antibody; GTP-binding protein overexpressed in skeletal muscle antibody; kinase-inducible Ras-like protein antibody; KIR antibody; MGC26294 antibody; OTTHUMP00000226600 antibody; OTTHUMP00000226601 antibody; RAS-like protein KIR antibody; GEM (aa34-46); anti-GEM antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: QKEPHQYSHRNRH
• Sequence Length: 296

• Applicable Applications for anti-GEM antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 16000.; Western Blot: In transfected HEK293 transiently expressing Human recombinant GEM bands of approx.35+45kDa are observed. These bands are not observed in the transfected HEK293 with empty vector and the antibody does not cross-react to related protein such as Rem, Rem2L or Rad. Data provided by Prof. R Andres, Univ. Kentucky, USA. A similar banding pattern we observed in Human Spleen. The calculated molecular size is 33.9kDa according to NP_005252.1. Recommended concentration: 1-3ug/ml.
• Immunogen: Peptide with sequence C-QKEPHQYSHRNRH, from the internal region of the protein sequence according to NP_005252.1.
• Epitope: Internal region
• Note: Reported variants represent identical protein: NP_005252.1, NP_859053.1
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

(HEK293 lysate overexpressing full-length Human GEM (HA tagged), mock-transfected HEK293 (EV) and HEK293 transiently expressing GEM-related genes (Rem, Rem2L and Rad) probed with (1ug/ml). The same lysates probed with anti-HA tag antibody in the lower panel.)

Western Blot (WB)

((1ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 4885263
• NCBI GeneID: 2669
• NCBI Accession #: NP_005252.1
• NCBI GenBank Nucleotide #: NM_005261.3
• Molecular Weight: 33,949 Da
• NCBI Official Full Name: GTP-binding protein GEM
• NCBI Official Synonym Full Names: GTP binding protein overexpressed in skeletal muscle
• NCBI Official Symbol: GEM
• NCBI Official Synonym Symbols: KIR
• NCBI Protein Information: GTP-binding protein GEM
• UniProt Protein Name: GTP-binding protein GEM
• Protein Family: Geminin
• UniProt Gene Name: GEM
• UniProt Synonym Gene Names: KIR
• UniProt Entry Name: GEM_HUMAN

NCBI Description

The protein encoded by this gene belongs to the RAD/GEM family of GTP-binding proteins. It is associated with the inner face of the plasma membrane and could play a role as a regulatory protein in receptor-mediated signal transduction. Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

GEM: Could be a regulatory protein, possibly participating in receptor-mediated signal transduction at the plasma membrane. Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity. Belongs to the small GTPase superfamily. RGK family.

Protein type: G protein, monomeric, RGK; G protein; G protein, monomeric

Chromosomal Location of Human Ortholog: 8q13-q21

Cellular Component: internal side of plasma membrane; midbody; nucleus; spindle midzone

Molecular Function: calmodulin binding; GDP binding; GTP binding; GTPase activity; magnesium ion binding; protein binding

Biological Process: cell surface receptor linked signal transduction; chromosome organization and biogenesis; immune response; metaphase plate congression; mitosis; protein transport; signal transduction; small GTPase mediated signal transduction