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Western Blot (WB) ((0.5ug/ml) staining of Mouse Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat Mboat4 Polyclonal Antibody | anti-Mboat4 antibody

Goat anti-Mboat4 (mouse) Antibody

Gene Names
Mboat4; GOAT; Gm171
Reactivity
Tested: Mouse; Expected from sequence similarity: Human, Mouse, Pig, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Mboat4; Polyclonal Antibody; Goat anti-Mboat4 (mouse) Antibody; Goat Anti-GOAT / MBOAT4 Antibody; membrane bound O-acyltransferase domain containing 4; GOAT; Gm171; O-acyltransferase (membrane bound) domain containing 4; O-acyltransferase domain-containing protein 4; ghrelin O-acyltransferase; membrane-bound O-acyltransferase domain-containing; Mboat4 antibody; membrane bound O-acyltransferase domain containing 4 antibody; GOAT antibody; Gm171 antibody; O-acyltransferase (membrane bound) domain containing 4 antibody; O-acyltransferase domain-containing protein 4 antibody; ghrelin O-acyltransferase antibody; membrane-bound O-acyltransferase domain-containing antibody; Mboat4 (mouse); anti-Mboat4 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Mouse; Expected from sequence similarity: Human, Mouse, Pig, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
CHLGLHYTEYYLGEP
Sequence Length
435
Applicable Applications for anti-Mboat4 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunogen
Peptide with sequence CHLGLHYTEYYLGEP, from the internal region of the protein sequence according to NP_001119786.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.5ug/ml) staining of Mouse Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((0.5ug/ml) staining of Mouse Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
49,879 Da
NCBI Official Full Name
ghrelin O-acyltransferase
NCBI Official Synonym Full Names
membrane bound O-acyltransferase domain containing 4
NCBI Official Symbol
Mboat4
NCBI Official Synonym Symbols
GOAT; Gm171
NCBI Protein Information
ghrelin O-acyltransferase
UniProt Protein Name
Ghrelin O-acyltransferase
Protein Family
UniProt Gene Name
Mboat4
UniProt Synonym Gene Names
Gm171; Goat; O-acyltransferase domain-containing protein 4
UniProt Entry Name
MBOA4_MOUSE

Uniprot Description

MBOAT4: Mediates the octanoylation of ghrelin at 'Ser-3'. Can use a variety of fatty acids as substrates including octanoic acid, decanoic acid and tetradecanoic acid. Belongs to the membrane-bound acyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Endoplasmic reticulum; EC 2.3.1.-; Membrane protein, integral; Transferase; Membrane protein, multi-pass

Cellular Component: endoplasmic reticulum; integral to endoplasmic reticulum membrane; integral to membrane; membrane

Molecular Function: 3-hydroxybutyryl-CoA thiolase activity; 3-ketopimelyl-CoA thiolase activity; acetyltransferase activity; acyl-CoA N-acyltransferase activity; acylglycerol O-acyltransferase activity; azetidine-2-carboxylic acid acetyltransferase activity; benzoyl acetate-CoA thiolase activity; C-acyltransferase activity; C-palmitoyltransferase activity; carnitine O-acyltransferase activity; dihydrolipoamide branched chain acyltransferase activity; dihydrolipoamide S-acyltransferase activity; glucosaminyl-phosphotidylinositol O-acyltransferase activity; keto acid formate lyase activity; L-2-aminoadipate N-acetyltransferase activity; malonyltransferase activity; myristoyltransferase activity; N-acetyltransferase activity; N-acyltransferase activity; N-palmitoyltransferase activity; N-succinyltransferase activity; O-acetyltransferase activity; O-acyltransferase activity; O-octanoyltransferase activity; O-palmitoyltransferase activity; O-sinapoyltransferase activity; O-succinyltransferase activity; octanoyltransferase activity; palmitoleoyl [acyl-carrier-protein]-dependent acyltransferase activity; palmitoyltransferase activity; peptidyl-lysine N6-myristoyltransferase activity; peptidyl-lysine N6-palmitoyltransferase activity; protein-cysteine S-acyltransferase activity; protein-cysteine S-myristoyltransferase activity; Ras palmitoyltransferase activity; S-acetyltransferase activity; S-acyltransferase activity; S-malonyltransferase activity; S-succinyltransferase activity; serine O-acyltransferase activity; sinapoyltransferase activity; sterol O-acyltransferase activity; succinyltransferase activity; transferase activity; transferase activity, transferring acyl groups

Biological Process: peptidyl-serine octanoylation

Research Articles on Mboat4

Similar Products

Product Notes

The Mboat4 mboat4 (Catalog #AAA422778) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Mboat4 (mouse) Antibody reacts with Tested: Mouse; Expected from sequence similarity: Human, Mouse, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's Mboat4 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Researchers should empirically determine the suitability of the Mboat4 mboat4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: CHLGLHYTEY YLGEP. It is sometimes possible for the material contained within the vial of "Mboat4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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