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Western Blot (WB) ((1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat Htr1a Polyclonal Antibody | anti-Htr1a antibody

Goat anti-Htr1a (mouse) Antibody

Gene Names
Htr1a; Gpcr18
Reactivity
Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Htr1a; Polyclonal Antibody; Goat anti-Htr1a (mouse) Antibody; Goat Anti-Serotonin receptor 1A / HTR1A Antibody; 5-hydroxytryptamine (serotonin) receptor 1A; Gpcr18; 5-HT-1A; 5-HT1A receptor; 5-hydroxytryptamine receptor 1A; G-protein coupled receptor 18; serotonin receptor 1A; Htr1a antibody; 5-hydroxytryptamine (serotonin) receptor 1A antibody; Gpcr18 antibody; 5-HT-1A antibody; 5-HT1A receptor antibody; 5-hydroxytryptamine receptor 1A antibody; G-protein coupled receptor 18 antibody; serotonin receptor 1A antibody; Htr1a (mouse); anti-Htr1a antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
ERKNERTAEAKRK
Sequence Length
421
Applicable Applications for anti-Htr1a antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 45kDa band observed in Mouse Brain lysates (calculated MW of 46.2kDa according to Mouse NP_032334.2). Recommended concentration: 1-3ug/ml. Additional bands of 75 and 18kDa of unknown identity were consistently observed. These bands were successfully blocked by incubation with the immunizing peptide.
Immunogen
Peptide with sequence C-ERKNERTAEAKRK, from the internal region of the protein sequence according to NP_032334.2.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,176 Da
NCBI Official Full Name
5-hydroxytryptamine receptor 1A
NCBI Official Synonym Full Names
5-hydroxytryptamine (serotonin) receptor 1A
NCBI Official Symbol
Htr1a
NCBI Official Synonym Symbols
Gpcr18
NCBI Protein Information
5-hydroxytryptamine receptor 1A
UniProt Protein Name
5-hydroxytryptamine receptor 1A
UniProt Gene Name
Htr1a
UniProt Synonym Gene Names
Gpcr18; 5-HT-1A; 5-HT1A
UniProt Entry Name
5HT1A_MOUSE

Uniprot Description

5-HT(1A): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Belongs to the G-protein coupled receptor 1 family. 5- hydroxytryptamine receptor subfamily. HTR1A sub-subfamily.

Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: axon hillock; cell soma; integral to membrane; integral to plasma membrane; membrane; plasma membrane

Molecular Function: drug binding; G-protein alpha-subunit binding; G-protein coupled receptor activity; serotonin binding; serotonin receptor activity; signal transducer activity

Biological Process: behavior; behavioral fear response; cell proliferation; G-protein coupled receptor protein signaling pathway; positive regulation of microtubule depolymerization; regulation of behavior; regulation of dopamine metabolic process; regulation of hormone secretion; regulation of serotonin secretion; serotonin metabolic process; serotonin receptor signaling pathway; serotonin receptor, adenylate cyclase inhibiting pathway; signal transduction; vasoconstriction

Research Articles on Htr1a

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Product Notes

The Htr1a htr1a (Catalog #AAA422752) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Htr1a (mouse) Antibody reacts with Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Htr1a can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 45kDa band observed in Mouse Brain lysates (calculated MW of 46.2kDa according to Mouse NP_032334.2). Recommended concentration: 1-3ug/ml. Additional bands of 75 and 18kDa of unknown identity were consistently observed. These bands were successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the Htr1a htr1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ERKNERTAEA KRK. It is sometimes possible for the material contained within the vial of "Htr1a, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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