Western Blot (WB)
((1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat GAD25/GAD67 Polyclonal Antibody | anti-GAD1 antibody
Goat anti-GAD25/GAD67 Antibody
Western Blot: Approx 70kDa band observed in Mouse Brain lysates (calculated MW of 66.6kDa according to Mouse NP_032103.2). Recommended concentration: 1-3ug/ml.
Western Blot (WB)
((1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008]
Uniprot Description
GAD67: Catalyzes the production of GABA. Homodimer. Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. Belongs to the group II decarboxylase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 4.1.1.15; Other Amino Acids Metabolism - taurine and hypotaurine; Lyase; Mitochondrial; Amino Acid Metabolism - alanine, aspartate and glutamate; Other Amino Acids Metabolism - beta-alanine; Carbohydrate Metabolism - butanoate
Chromosomal Location of Human Ortholog: 2q31
Cellular Component: intracellular; plasma membrane; presynaptic active zone; vesicle membrane
Molecular Function: glutamate binding; glutamate decarboxylase activity; protein binding; protein heterodimerization activity; protein N-terminus binding; pyridoxal phosphate binding
Biological Process: gamma-aminobutyric acid biosynthetic process; glutamate catabolic process; glutamate decarboxylation to succinate; neurotransmitter biosynthetic process; neurotransmitter secretion; protein-pyridoxal-5-phosphate linkage; response to drug; synaptic transmission
Disease: Cerebral Palsy, Spastic Quadriplegic, 1
Research Articles on GAD1
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Product Notes
The GAD1 gad1 (Catalog #AAA422705) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-GAD25/GAD67 Antibody reacts with Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Pig, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's GAD25/GAD67 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx 70kDa band observed in Mouse Brain lysates (calculated MW of 66.6kDa according to Mouse NP_032103.2). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the GAD1 gad1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QRTNSLEEKS R. It is sometimes possible for the material contained within the vial of "GAD25/GAD67, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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