Goat ABCB8 Polyclonal Antibody | anti-ABCB8 antibody

Goat anti-ABCB8 Antibody

Cat. Num. AAA422177

• Gene Names: ABCB8; MABC1; M-ABC1; EST328128
• Reactivity: Expected from sequence similarity: Human
• Applications: ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: ABCB8; Polyclonal Antibody; Goat anti-ABCB8 Antibody; ATP-binding cassette; sub-family B (MDR/TAP); member 8; EST328128; M-ABC1; MABC1; sub-family B; mitochondrial ABC protein; ABCB8 antibody; member 8 antibody; EST328128 antibody; M-ABC1 antibody; MABC1 antibody; mitochondrial ABC protein antibody; anti-ABCB8 antibody

• Host: Goat
• Reactivity: Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: KKPEGPRSHQHK
• Sequence Length: 718

• Applicable Applications for anti-ABCB8 antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 128000.; Western Blot: Preliminary experiments gave an approx 22kDa band in lysates of cell lines HeLa and Jurkat after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 78.1kDa according to NP_009119.2. The 22kDa band was successfully blocked by incubation with the immunizing peptide.
• Immunogen: Peptide with sequence C-KKPEGPRSHQHK, from the C Terminus of the protein sequence according to NP_009119.2.
• Epitope: C Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 156105685
• NCBI GeneID: 11194
• NCBI Accession #: NP_009119.2
• NCBI GenBank Nucleotide #: NM_007188.4
• Molecular Weight: 59,383 Da
• NCBI Official Full Name: ATP-binding cassette sub-family B member 8, mitochondrial isoform b
• NCBI Official Synonym Full Names: ATP binding cassette subfamily B member 8
• NCBI Official Symbol: ABCB8
• NCBI Official Synonym Symbols: MABC1; M-ABC1; EST328128
• NCBI Protein Information: ATP-binding cassette sub-family B member 8, mitochondrial
• UniProt Protein Name: ATP-binding cassette sub-family B member 8, mitochondrial
• Protein Family: ATP-binding cassette sub-family
• UniProt Gene Name: ABCB8
• UniProt Synonym Gene Names: MABC1; M-ABC1
• UniProt Entry Name: ABCB8_HUMAN

NCBI Description

This nuclear gene encodes a multi-pass membrane protein that is targeted to the mitochondrial inner membrane. The encoded protein is an ATP-dependent transporter that may mediate the passage of organic and inorganic molecules out of the mitochondria. Loss of function of the related gene in mouse results in a disruption of iron homeostasis between the mitochondria and cytosol. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

ABCB8: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. The function of this half-transporter has not yet been determined; however, it may involve the compartmentalization and transport of heme, as well as peptides, from the mitochondria to the nucleus and cytosol. This protein may also play a role in the transport of phospholipids into mitochondrial membranes. [provided by RefSeq, Jul 2008]

Protein type: Hydrolase; Mitochondrial; Membrane protein, multi-pass; Membrane protein, integral; Transporter; Transporter, ABC family

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: ATP-binding cassette (ABC) transporter complex; membrane; mitochondrial envelope; mitochondrial inner membrane; mitochondrion; nucleolus; nucleus

Molecular Function: ATP binding; ATPase activity, coupled to transmembrane movement of substances; transporter activity

Biological Process: metabolic process; transmembrane transport; transport