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Western Blot (WB) ((0. 01ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat TM4SF3/TSPAN8 Polyclonal Antibody | anti-TSPAN8 antibody

Goat anti-TM4SF3/TSPAN8 Antibody

Gene Names
TSPAN8; CO-029; TM4SF3
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
TM4SF3/TSPAN8; Polyclonal Antibody; Goat anti-TM4SF3/TSPAN8 Antibody; TSPAN8; tetraspanin 8; CO-029; TM4SF3; transmembrane 4 superfamily member 3; tumor-associated antigen CO-029; TSPAN8 antibody; tetraspanin 8 antibody; CO-029 antibody; TM4SF3 antibody; transmembrane 4 superfamily member 3 antibody; tumor-associated antigen CO-029 antibody; TM4SF3 / TSPAN8; anti-TSPAN8 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
QRPCQSYNGKQVYKE
Sequence Length
237
Applicable Applications for anti-TSPAN8 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 24kDa band observed in Human Colon lysates (calculated MW of 26. 0kDa according to NP_004607.1). Recommended concentration: 0.01-0.03ug/ml.
Immunogen
Peptide with sequence QRPCQSYNGKQVYKE, from the internal region of the protein sequence according to NP_004607.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0. 01ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((0. 01ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,044 Da
NCBI Official Full Name
tetraspanin-8
NCBI Official Synonym Full Names
tetraspanin 8
NCBI Official Symbol
TSPAN8
NCBI Official Synonym Symbols
CO-029; TM4SF3
NCBI Protein Information
tetraspanin-8
UniProt Protein Name
Tetraspanin-8
UniProt Gene Name
TSPAN8
UniProt Synonym Gene Names
TM4SF3; Tspan-8
UniProt Entry Name
TSN8_HUMAN

NCBI Description

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This gene is expressed in different carcinomas. The use of alternate polyadenylation sites has been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TSPAN8: is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This gene is expressed in different carcinomas. The use of alternate polyadenylation sites has been found for this gene. [provided by RefSeq, Jul 2008]

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q14.1-q21.1

Cellular Component: integral to plasma membrane

Molecular Function: integrin binding

Biological Process: cell surface receptor linked signal transduction; negative regulation of blood coagulation

Research Articles on TSPAN8

Similar Products

Product Notes

The TSPAN8 tspan8 (Catalog #AAA422114) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-TM4SF3/TSPAN8 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's TM4SF3/TSPAN8 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 24kDa band observed in Human Colon lysates (calculated MW of 26. 0kDa according to NP_004607.1). Recommended concentration: 0.01-0.03ug/ml. Researchers should empirically determine the suitability of the TSPAN8 tspan8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QRPCQSYNGK QVYKE. It is sometimes possible for the material contained within the vial of "TM4SF3/TSPAN8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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