Western Blot (WB)
((0.3ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Bin3 Polyclonal Antibody | anti-MEPCE antibody
Goat anti-Bin3 Antibody
Western Blot: Approx.75kDa band observed in lysates of cell line K562 (calculated MW of 74.4kDa according to NP_062552.2). Recommended concentration: 0.3-1ug/ml.
Western Blot (WB)
((0.3ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
Uniprot Description
BCDIN3: a CDK9-associated enzyme that forms the distinctive gamma-methylphosphate cap structure of 7SK, a noncoding RNA that regulates CDK9 activity. An adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to its stabilization. Component of the 7SK snRNP complex that contains P-TEFb (composed of CDK9 and CCNT1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Expressed in chronic myeloid leukemia cells, adrenal gland, brain, cerebellum, kidney, lung, mammary gland and testis. Weakly or not expressed in other tissues. Contains a Bin3 domain found in a number of protein methyltransferases that modify RNA-binding proteins.
Protein type: Transcription regulation; Methyltransferase; EC 2.1.1.-; RNA processing; EC 2.1.1.
Chromosomal Location of Human Ortholog: 7q22.1
Molecular Function: RNA methyltransferase activity; S-adenosylmethionine-dependent methyltransferase activity
Biological Process: negative regulation of transcription from RNA polymerase II promoter; RNA methylation; snRNA metabolic process; snRNA modification
Research Articles on MEPCE
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Product Notes
The MEPCE mepce (Catalog #AAA422093) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Bin3 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's Bin3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx.75kDa band observed in lysates of cell line K562 (calculated MW of 74.4kDa according to NP_062552.2). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the MEPCE mepce for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: TPHNTSKGFQ RP. It is sometimes possible for the material contained within the vial of "Bin3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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