Thrombin Receptor Recombinant Protein | F2R recombinant protein

Thrombin Receptor Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121451

• Gene Names: F2R; TR; HTR; CF2R; PAR1; PAR-1
• Purity: >90% as determined by SDS-PAGE
• Synonyms: Thrombin Receptor; Thrombin Receptor Protein; Human; Recombinant (His Tag); Human F2R/Coagulation factor II receptor/Thrombin Receptor Protein (His Tag); CF2R Protein; HTR Protein; PAR-1 Protein; PAR1 Protein; TR Protein; coagulation factor II (thrombin) receptor; F2R recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Thr102

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Arg 27
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human F2R (NP_001983.2)(Met1-Thr102) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 166362740
• NCBI GeneID: 2149
• NCBI Accession #: NP_001983.2
• NCBI GenBank Nucleotide #: NM_001992.3
• UniProt Accession #: P25116; Q53XV0; Q96RF7; Q9BUN4
• Molecular Weight: 47,441 Da
• NCBI Official Full Name: proteinase-activated receptor 1
• NCBI Official Synonym Full Names: coagulation factor II (thrombin) receptor
• NCBI Official Symbol: F2R
• NCBI Official Synonym Symbols: TR; HTR; CF2R; PAR1; PAR-1
• NCBI Protein Information: proteinase-activated receptor 1; protease-activated receptor 1
• UniProt Protein Name: Proteinase-activated receptor 1
• Protein Family: Proteinase-activated receptor
• UniProt Gene Name: F2R
• UniProt Synonym Gene Names: CF2R; PAR1; TR; PAR-1
• UniProt Entry Name: PAR1_HUMAN

NCBI Description

Coagulation factor II receptor is a 7-transmembrane receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. F2R is a G-protein coupled receptor family member. [provided by RefSeq, Jul 2008]

Uniprot Description

PAR1: a G-protein coupled high-affinity receptor for activated thrombin or trypsin. Coupled to G proteins that stimulate phosphoinositide hydrolysis. Coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelet activation and in vascular development.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR; Cell development/differentiation

Chromosomal Location of Human Ortholog: 5q13

Cellular Component: Golgi apparatus; postsynaptic membrane; integral to plasma membrane; early endosome; extracellular region; plasma membrane; caveola; neuromuscular junction

Molecular Function: G-protein coupled receptor activity; protein binding; G-protein alpha-subunit binding; G-protein beta-subunit binding; receptor binding; thrombin receptor activity

Biological Process: elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of blood coagulation; negative regulation of glomerular filtration; activation of MAPKK activity; positive regulation of transcription, DNA-dependent; positive regulation of collagen biosynthetic process; positive regulation of caspase activity; positive regulation of JAK-STAT cascade; response to lipopolysaccharide; negative regulation of cell proliferation; connective tissue replacement during inflammatory response; elevation of cytosolic calcium ion concentration; platelet dense granule organization and biogenesis; positive regulation of MAPKKK cascade; regulation of interleukin-1 beta production; protein kinase C activation; positive regulation of cell proliferation; response to wounding; negative regulation of neuron apoptosis; positive regulation of smooth muscle contraction; establishment of synaptic specificity at neuromuscular junction; inflammatory response; STAT protein nuclear translocation; caspase activation; platelet activation; anatomical structure morphogenesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of Rho protein signal transduction; positive regulation of phosphoinositide 3-kinase cascade; tyrosine phosphorylation of STAT protein; G-protein coupled receptor protein signaling pathway; homeostasis of number of cells within a tissue; release of sequestered calcium ion into cytosol; regulation of blood coagulation; positive regulation of vasoconstriction; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of release of sequestered calcium ion into cytosol; regulation of sensory perception of pain; positive regulation of calcium ion transport; blood coagulation; positive regulation of cell migration