Goat HEM1 Polyclonal Antibody | anti-NCKAP1L antibody
Goat anti-HEM1 Antibody
Western Blot: Preliminary experiments gave an approx.50kDa band in Human Tonsil and Thymus lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 128kDa according to NP_005328.2. The 50kDa band was successfully blocked by incubation with the immunizing peptide.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the HEM family of tissue-specific transmembrane proteins which are highly conserved from invertebrates through mammals. This gene is only expressed in hematopoietic cells. The encoded protein is a part of the Scar/WAVE complex which plays an important role in regulating cell shape in both metazoans and plants. Alternatively spliced transcript variants encoding different isoforms have been found.[provided by RefSeq, May 2010]
Uniprot Description
HEM1: a component of the Scar/WAVE complex that plays an important role in regulating cell shape and motility. Found at elevated levels in cells of hematopoietic lineage. Localizes to propagating waves at the leading edge of a motile human neutrophils. The complex stimulates actin assembly; polymerized actin is reciprocally required to remove the complex from the membrane. Overexpression is associated with poor outcome in B-cell chronic lymphocytic leukemia patients.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 12q13.1
Cellular Component: cytosol; integral to plasma membrane; membrane
Molecular Function: GTPase activator activity; protein binding; protein complex binding; protein kinase activator activity
Biological Process: activation of protein kinase activity; B cell homeostasis; B cell receptor signaling pathway; chemotaxis; cortical actin cytoskeleton organization and biogenesis; erythrocyte development; maintenance of cell polarity; myeloid cell homeostasis; negative regulation of apoptosis; negative regulation of interleukin-17 production; negative regulation of interleukin-6 production; neutrophil chemotaxis; positive regulation of actin filament polymerization; positive regulation of B cell differentiation; positive regulation of B cell proliferation; positive regulation of CD4-positive, alpha beta T cell differentiation; positive regulation of CD8-positive, alpha-beta T cell differentiation; positive regulation of cell adhesion mediated by integrin; positive regulation of erythrocyte differentiation; positive regulation of gamma-delta T cell differentiation; positive regulation of GTPase activity; positive regulation of lymphocyte differentiation; positive regulation of phagocytosis, engulfment; positive regulation of phosphorylation; positive regulation of T cell proliferation; protein complex assembly; response to drug; T cell homeostasis; vascular endothelial growth factor receptor signaling pathway
Research Articles on NCKAP1L
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Product Notes
The NCKAP1L nckap1l (Catalog #AAA422020) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-HEM1 Antibody reacts with Expected from sequence similarity: Human, Mouse, Rat, Dog, Horse and may cross-react with other species as described in the data sheet. AAA Biotech's HEM1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Preliminary experiments gave an approx.50kDa band in Human Tonsil and Thymus lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 128kDa according to NP_005328.2. The 50kDa band was successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the NCKAP1L nckap1l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RHTENVTKTK TPE. It is sometimes possible for the material contained within the vial of "HEM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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