Goat TREX2 Polyclonal Antibody | anti-TREX2 antibody

Goat anti-TREX2 Antibody

Cat. Num. AAA421675

• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: TREX2; Polyclonal Antibody; Goat anti-TREX2 Antibody; three prime repair exonuclease 2; 3'-5' exonuclease; three prime repair exonuclease 2 antibody; 3\1-5\1 exonuclease antibody; anti-TREX2 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: HRSSLENPEHDES
• Sequence Length: 236

• Applicable Applications for anti-TREX2 antibody: Peptide ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 128000.; Immunohistochemistry: In paraffin embedded Human Breast shows nuclear staining in the luminal epithelial duct cells. Recommended concentration, 3-6ug/ml.; Western Blot: Preliminary experiments gave an approx 18kDa band in lysates of Jurkat, MOLT4 and U937 after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 25.9kDa according to NP_542432.2. The 18kDa band was successfully blocked by incubation with the immunizing peptide.
• Immunogen: Peptide with sequence C-HRSSLENPEHDES, from the internal region of the protein sequence according to NP_542432.2.
• Epitope: Internal region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

((3.8ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 63079718
• NCBI GeneID: 11219
• NCBI Accession #: NP_542432.2
• NCBI GenBank Nucleotide #: NM_080701.3
• Molecular Weight: 25,922 Da
• NCBI Official Full Name: three prime repair exonuclease 2
• NCBI Official Synonym Full Names: three prime repair exonuclease 2
• NCBI Official Symbol: TREX2
• NCBI Protein Information: three prime repair exonuclease 2
• UniProt Protein Name: Three prime repair exonuclease 2
• Protein Family: Three prime repair exonuclease
• UniProt Gene Name: TREX2
• UniProt Entry Name: TREX2_HUMAN

NCBI Description

This gene encodes a nuclear protein with 3' to 5' exonuclease activity. The encoded protein participates in double-stranded DNA break repair, and may interact with DNA polymerase delta. [provided by RefSeq, Nov 2012]

Uniprot Description

TREX2: Exonuclease with a preference for double stranded DNA with mismatched 3' termini. May play a role in DNA repair. Belongs to the exonuclease superfamily. TREX family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage; EC 3.1.11.2; Deoxyribonuclease

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: nucleus

Molecular Function: 3'-5'-exodeoxyribonuclease activity; exodeoxyribonuclease III activity; magnesium ion binding; nucleic acid binding; protein homodimerization activity

Biological Process: DNA metabolic process; DNA repair