Western Blot (WB)
((0.2ug/ml) staining of Rat Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat LASS3/CerS3 Polyclonal Antibody | anti-LASS3 antibody
Goat anti-LASS3/CerS3 Antibody
Western Blot: approx. 45 kDa band observed in Rat Testis lysates (calculated MW of 46.3 kDa according to Human NP_849164.2 and of 47.2 kDa according to Rat NP_001121033.1). Recommended concentration: 2-3ug/ml.
Western Blot (WB)
((0.2ug/ml) staining of Rat Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.2ug/ml) staining of Rat Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the ceramide synthase family of genes. The ceramide synthase enzymes regulate sphingolipid synthesis by catalyzing the formation of ceramides from sphingoid base and acyl-coA substrates. This family member is involved in the synthesis of ceramides with ultra-long-chain acyl moieties (ULC-Cers), important to the epidermis in its role in creating a protective barrier from the environment. The protein encoded by this gene has also been implicated in modification of the lipid structures required for spermatogenesis. Mutations in this gene have been associated with male fertility defects, and epidermal defects, including ichthyosis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]
Uniprot Description
Lass3: Has (dihydro)ceramide synthesis activity with relatively broad substrate specificity, but a preference for C18:0 and other middle- to long-chain fatty acyl-CoAs. It is crucial for the synthesis of very long-chain ceramides in the epidermis, to maintain epidermal lipid homeostasis and terminal differentiation.
Protein type: DNA-binding; Membrane protein, multi-pass; Membrane protein, integral; EC 2.3.1.24
Chromosomal Location of Human Ortholog: 15q26.3
Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to membrane; nuclear membrane
Molecular Function: DNA binding; sphingosine N-acyltransferase activity
Biological Process: ceramide biosynthetic process; keratinocyte differentiation; sphingolipid biosynthetic process
Disease: Ichthyosis, Congenital, Autosomal Recessive 9
Research Articles on LASS3
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Product Notes
The LASS3 cers3 (Catalog #AAA421629) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-LASS3/CerS3 Antibody reacts with Tested: Rat; Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's LASS3/CerS3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: approx. 45 kDa band observed in Rat Testis lysates (calculated MW of 46.3 kDa according to Human NP_849164.2 and of 47.2 kDa according to Rat NP_001121033.1). Recommended concentration: 2-3ug/ml. Researchers should empirically determine the suitability of the LASS3 cers3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RAERHLIPNG QHGH. It is sometimes possible for the material contained within the vial of "LASS3/CerS3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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