Western Blot (WB)
((1ug/ml) staining of Human Brain (hippocampus) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat PACSIN1 Polyclonal Antibody | anti-PACSIN1 antibody
Goat anti-PACSIN1 Antibody
Gene Names
PACSIN1; SDPI
Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog, Pig, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
PACSIN1; Polyclonal Antibody; Goat anti-PACSIN1 Antibody; protein kinase C and casein kinase substrate in neurons 1; KIAA1379; SDPI; syndapin I; PACSIN1 antibody; protein kinase C and casein kinase substrate in neurons 1 antibody; KIAA1379 antibody; SDPI antibody; syndapin I antibody; anti-PACSIN1 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog, Pig, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
SSSYDEASLAPEET
Sequence Length
444
Applicable Applications for anti-PACSIN1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 50kDa band observed in Human Brain (hippocampus) lysates (calculated MW of 51.1kDa according to NP_065855.1). Recommended concentration: 0. 03-0.1ug/ml. An additional band of unknown identity was also consistently observed at 37kDa.
Western Blot: Approx 50kDa band observed in Human Brain (hippocampus) lysates (calculated MW of 51.1kDa according to NP_065855.1). Recommended concentration: 0. 03-0.1ug/ml. An additional band of unknown identity was also consistently observed at 37kDa.
Immunogen
Peptide with sequence SSSYDEASLAPEET-C, from the N Terminus of the protein sequence according to NP_065855.1.
Epitope
N Terminus
Note
Reported variants represent identical protein (NP_065855.1; NP_001186512.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((1ug/ml) staining of Human Brain (hippocampus) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Human Brain (hippocampus) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,966 Da
NCBI Official Full Name
protein kinase C and casein kinase substrate in neurons protein 1
NCBI Official Synonym Full Names
protein kinase C and casein kinase substrate in neurons 1
NCBI Official Symbol
PACSIN1
NCBI Official Synonym Symbols
SDPI
NCBI Protein Information
protein kinase C and casein kinase substrate in neurons protein 1
UniProt Protein Name
Protein kinase C and casein kinase substrate in neurons protein 1
UniProt Gene Name
PACSIN1
UniProt Synonym Gene Names
KIAA1379
UniProt Entry Name
PACN1_HUMAN
Uniprot Description
PACSIN1: May play a role in vesicle formation and transport. Belongs to the PACSIN family.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 6p21.3
Cellular Component: cell junction; COPI-coated vesicle; cytoplasm; cytoplasmic vesicle membrane; cytosol; endosome; myelin sheath; nerve terminal; perinuclear region of cytoplasm; plasma membrane; synapse
Molecular Function: cytoskeletal protein binding; phospholipid binding; protein binding
Biological Process: actin filament organization; negative regulation of endocytosis; neurite morphogenesis; regulation of endocytosis; synaptic vesicle endocytosis
Research Articles on PACSIN1
Similar Products
Product Notes
The PACSIN1 pacsin1 (Catalog #AAA421379) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PACSIN1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's PACSIN1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 50kDa band observed in Human Brain (hippocampus) lysates (calculated MW of 51.1kDa according to NP_065855.1). Recommended concentration: 0. 03-0.1ug/ml. An additional band of unknown identity was also consistently observed at 37kDa. Researchers should empirically determine the suitability of the PACSIN1 pacsin1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SSSYDEASLA PEET. It is sometimes possible for the material contained within the vial of "PACSIN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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