Western Blot (WB)
((1ug/ml) staining of human bone marrow lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat LXR beta/NR1H2 Polyclonal Antibody | anti-NR1H2 antibody
Goat anti-LXR beta/NR1H2 Antibody
Immunohistochemistry: In paraffin embedded Human Spleen shows nucleolar staining. Recommended concentration, 1-2ug/ml.
Western Blot: Experiments gave a band at approx 80kDa and a weak band at appr. 60-kDa in human bone marrow lysate after 1ug/ml antibody staining. These bands correspond to earlier findings with different antibodies (PMID: 12393874-60kDa and other unpublished antibodies-80kDa). This protein has a calculated MW of 51. 0kDa according to NP_009052. Recommended concentration: 1-3ug/ml.
Western Blot (WB)
((1ug/ml) staining of human bone marrow lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of human bone marrow lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((3ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
The liver X receptors, LXRA (NR1H3; MIM 602423) and LXRB, form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. The inducible LXRA is highly expressed in liver, adrenal gland, intestine, adipose tissue, macrophages, lung, and kidney, whereas LXRB is ubiquitously expressed. Ligand-activated LXRs form obligate heterodimers with retinoid X receptors (RXRs; see MIM 180245) and regulate expression of target genes containing LXR response elements (summary by Korf et al., 2009 [PubMed 19436111]).[supplied by OMIM, Jan 2010]
Uniprot Description
LXR-beta: Orphan receptor. Binds preferentially to double-stranded oligonucleotide direct repeats having the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). Regulates cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Forms a heterodimer with RXR. Ubiquitous. Belongs to the nuclear hormone receptor family. NR1 subfamily.
Protein type: Nuclear receptor
Chromosomal Location of Human Ortholog: 19q13.3
Cellular Component: cytoplasm; nucleoplasm; nucleus
Molecular Function: apolipoprotein A-I receptor binding; ATPase binding; DNA binding; ligand-dependent nuclear receptor activity; protein binding; retinoid X receptor binding; steroid hormone receptor activity; zinc ion binding
Biological Process: cellular lipid metabolic process; cholesterol homeostasis; negative regulation of lipid transport; negative regulation of pinocytosis; negative regulation of proteolysis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of cellular protein metabolic process; positive regulation of cholesterol transport; positive regulation of fatty acid biosynthetic process; positive regulation of lipoprotein lipase activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; retinoic acid receptor signaling pathway; steroid hormone mediated signaling; transcription initiation from RNA polymerase II promoter
Research Articles on NR1H2
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Product Notes
The NR1H2 nr1h2 (Catalog #AAA421375) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-LXR beta/NR1H2 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's LXR beta/NR1H2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 64000. Immunohistochemistry: In paraffin embedded Human Spleen shows nucleolar staining. Recommended concentration, 1-2ug/ml. Western Blot: Experiments gave a band at approx 80kDa and a weak band at appr. 60-kDa in human bone marrow lysate after 1ug/ml antibody staining. These bands correspond to earlier findings with different antibodies (PMID: 12393874-60kDa and other unpublished antibodies-80kDa). This protein has a calculated MW of 51. 0kDa according to NP_009052. Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the NR1H2 nr1h2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SSPTTSSLDT PLPGC. It is sometimes possible for the material contained within the vial of "LXR beta/NR1H2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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