Immunohistochemistry (IHC)
(Peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ADD1 (Ab-726) antibody.)
ADD1 Antibody | anti-ADD1 antibody
ADD1 (Ab-726) Antibody
Gene Names
ADD1; ADDA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
ADD1; ADD1 (Ab-726) Antibody; anti-ADD1 antibody
Reactivity
Human, Mouse, Rat
Specificity
ADD1 (Ab-726) antibody detects endogenous levels of total ADD1 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
737
Applicable Applications for anti-ADD1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:200
IHC: 1:50~1:100
IF: 1:100~1:200
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human ADD1 around the phosphorylation site of serine 726 (T-P-S P-F-L).
Preparation and Storage
Store at-20 degree C for 1 year
Immunohistochemistry (IHC)
(Peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ADD1 (Ab-726) antibody.)
Immunohistochemistry (IHC)
(Peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ADD1 (Ab-726) antibody.)
Western Blot (WB)
(Western blot analysis of extract from HT-29 cells untreated or treated with Doxorubicin (1mM, 30min), using ADD1 (Ab-726) antibody and ADD1 (Phospho-Ser726) antibody.)
Western Blot (WB)
(Western blot analysis of extract from HT-29 cells untreated or treated with Doxorubicin (1mM, 30min), using ADD1 (Ab-726) antibody and ADD1 (Phospho-Ser726) antibody.)
Product Categories/Family for anti-ADD1 antibody
References
Pariser H, et al. Proc Natl Acad Sci USA 2005 Aug 30; 102(35): 12407-12 Tamaru S, et al. Biochem Biophys Res Commun 2005 Jul 01; 332(2): 347-51 Barkalow KL, et al. J Cell Biol 2003 May 12; 161(3): 557-70.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
73,333 Da
NCBI Official Full Name
alpha-adducin isoform a
NCBI Official Synonym Full Names
adducin 1
NCBI Official Symbol
ADD1
NCBI Official Synonym Symbols
ADDA
NCBI Protein Information
alpha-adducin
UniProt Protein Name
Alpha-adducin
Protein Family
UniProt Gene Name
ADD1
UniProt Synonym Gene Names
ADDA
NCBI Description
Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, and gamma). Adducin acts as a heterodimer of the related alpha, beta, or gamma subunits. The protein encoded by this gene represents the alpha subunit. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. [provided by RefSeq, Aug 2017]
Uniprot Description
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
Research Articles on ADD1
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Product Notes
The ADD1 add1 (Catalog #AAA856985) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The ADD1 (Ab-726) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ADD1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:200. Researchers should empirically determine the suitability of the ADD1 add1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADD1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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