Goat Neudesin/NENF Polyclonal Antibody | anti-NENF antibody
Goat anti-Neudesin/NENF Antibody
Gene Names
NENF; CIR2; SPUF; SCIRP10
Reactivity
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Applications
ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Neudesin/NENF; Polyclonal Antibody; Goat anti-Neudesin/NENF Antibody; NENF; neuron derived neurotrophic factor; CIR2; SCIRP10; SPUF; SCIRP10-related protein; Spinal cord injury related protein 10; cell growth-inhibiting protein 47 neudesin secreted protein of unknown function; Neudesin; NENF antibody; neuron derived neurotrophic factor antibody; CIR2 antibody; SCIRP10 antibody; SPUF antibody; SCIRP10-related protein antibody; Spinal cord injury related protein 10 antibody; cell growth-inhibiting protein 47 neudesin secreted protein of unknown function antibody; Neudesin antibody; Neudesin / NENF; anti-NENF antibody
Host
Goat
Reactivity
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
NEDGSPNLDFKP
Sequence Length
172
Applicable Applications for anti-NENF antibody
Peptide ELISA (EIA)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Preliminary experiments gave an approx 45kDa band in Human Brain (Cerebellum) lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 18.6kDa according to NP_037481.1 The 45kDa band was successfully blocked by incubation with the immunizing peptide.
Western Blot: Preliminary experiments gave an approx 45kDa band in Human Brain (Cerebellum) lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 18.6kDa according to NP_037481.1 The 45kDa band was successfully blocked by incubation with the immunizing peptide.
Immunogen
Peptide with sequence C-NEDGSPNLDFKP, from the internal region (near the C Terminus) of the protein sequence according to NP_037481.1.
Epitope
Internal region (near the C Terminus)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,856 Da
NCBI Official Full Name
neudesin
NCBI Official Synonym Full Names
neudesin neurotrophic factor
NCBI Official Symbol
NENF
NCBI Official Synonym Symbols
CIR2; SPUF; SCIRP10
NCBI Protein Information
neudesin
UniProt Protein Name
Neudesin
Protein Family
UniProt Gene Name
NENF
UniProt Synonym Gene Names
CIR2; SPUF; SPUF protein
UniProt Entry Name
NENF_HUMAN
NCBI Description
This gene encodes a neurotrophic factor that may play a role in neuron differentiation and development. A pseudogene of this gene is found on chromosome 12. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2009]
Uniprot Description
NENF: Displays neurotrophic activity and activates phosphorylation of MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT in primary cultured neurons. Does not have mitogenic activity in primary cultured astrocytes. May play a role on neuronal differentiation and may have a transient effect on neural cell proliferation in neural precursor cells. Neurotrophic activity is enhanced by binding to heme. Belongs to the cytochrome b5 family. MAPR subfamily.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1q32.3
Cellular Component: endomembrane system; extracellular space; membrane; nucleoplasm; nucleus
Molecular Function: growth factor activity; metal ion binding
Biological Process: negative regulation of appetite; positive regulation of MAPKKK cascade
Research Articles on NENF
Similar Products
Product Notes
The NENF nenf (Catalog #AAA421096) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Neudesin/NENF Antibody reacts with Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's Neudesin/NENF can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Preliminary experiments gave an approx 45kDa band in Human Brain (Cerebellum) lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 18.6kDa according to NP_037481.1 The 45kDa band was successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the NENF nenf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: NEDGSPNLDF KP. It is sometimes possible for the material contained within the vial of "Neudesin/NENF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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