Flow Cytometry (FC/FACS)
(Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
Goat ALS2CR3/GRIF1 Polyclonal Antibody | anti-TRAK2 antibody
Goat anti-ALS2CR3/GRIF1 Antibody
Gene Names
TRAK2; GRIF1; MILT2; OIP98; CALS-C; GRIF-1; ALS2CR3
Reactivity
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow
Applications
ELISA, Flow Cytometry, Functional Assay
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
ALS2CR3/GRIF1; Polyclonal Antibody; Goat anti-ALS2CR3/GRIF1 Antibody; TRAK2; ALS2CR3; amyotrophic lateral sclerosis 2 (juvenile) chromosome region; candidate 3; GRIF1; CALS-C; GRIF-1; KIAA0549; likely ortholog of rat GABA(A) receptor-associated protein (GRIF-1); trafficking protein; kinesin binding 2; OIP98; TRAK2 antibody; ALS2CR3 antibody; candidate 3 antibody; GRIF1 antibody; CALS-C antibody; GRIF-1 antibody; KIAA0549 antibody; likely ortholog of rat GABA(A) receptor-associated protein (GRIF-1) antibody; kinesin binding 2 antibody; OIP98 antibody; ALS2CR3 / GRIF1; anti-TRAK2 antibody
Host
Goat
Reactivity
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
CTSSPKMGVLKED
Sequence Length
914
Applicable Applications for anti-TRAK2 antibody
Peptide ELISA (EIA); Flow Cytometry (FC/FACS)
Application Notes
Peptide ELISA: antibody detection limit dilution 1:32000.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Immunogen
Peptide with sequence CTSSPKMGVLKED, from the C Terminus of the protein sequence according to NP_055864.2.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,815 Da
NCBI Official Full Name
trafficking kinesin-binding protein 2
NCBI Official Synonym Full Names
trafficking kinesin protein 2
NCBI Official Symbol
TRAK2
NCBI Official Synonym Symbols
GRIF1; MILT2; OIP98; CALS-C; GRIF-1; ALS2CR3
NCBI Protein Information
trafficking kinesin-binding protein 2
UniProt Protein Name
Trafficking kinesin-binding protein 2
Protein Family
UniProt Gene Name
TRAK2
UniProt Synonym Gene Names
ALS2CR3; KIAA0549
UniProt Entry Name
TRAK2_HUMAN
Uniprot Description
TRAK2: an O-glycosylated protein that is widely expressed and that interacts with kinesin and O-GlcNAc transferase (OGT). Interacts with mitochondrial rho GTPase 2 (MIRO-2) forming a link between mitochondria and the trafficking apparatus of the microtubules.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 2q33
Cellular Component: cytoplasm; early endosome; mitochondrion; nucleus; plasma membrane
Molecular Function: enzyme binding; GABA receptor binding; protein binding; receptor binding
Biological Process: protein amino acid O-linked glycosylation; protein targeting; regulation of transcription from RNA polymerase II promoter
Research Articles on TRAK2
Similar Products
Product Notes
The TRAK2 trak2 (Catalog #AAA421079) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ALS2CR3/GRIF1 Antibody reacts with Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's ALS2CR3/GRIF1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA); Flow Cytometry (FC/FACS). Peptide ELISA: antibody detection limit dilution 1:32000. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. Researchers should empirically determine the suitability of the TRAK2 trak2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: CTSSPKMGVL KED. It is sometimes possible for the material contained within the vial of "ALS2CR3/GRIF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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