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Western Blot (WB) ((0.1ug/ml) staining of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat ZDHHC8 Polyclonal Antibody | anti-ZDHHC8 antibody

Goat anti-ZDHHC8 Antibody

Gene Names
ZDHHC8; DHHC8; ZNF378; ZDHHCL1
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
ZDHHC8; Polyclonal Antibody; Goat anti-ZDHHC8 Antibody; zinc finger; DHHC-type containing 8; ZDHHCL1; ZNF378; membrane-associated DHHC8 zinc finger protein; DHHC domain containing 8; DHHC domain like containing 1; ZDHHC8 antibody; DHHC-type containing 8 antibody; ZDHHCL1 antibody; ZNF378 antibody; membrane-associated DHHC8 zinc finger protein antibody; DHHC domain containing 8 antibody; DHHC domain like containing 1 antibody; anti-ZDHHC8 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
QRDHPQLKTPPSK
Sequence Length
765
Applicable Applications for anti-ZDHHC8 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunogen
Peptide with sequence C-QRDHPQLKTPPSK, from the internal region of the protein sequence according to NP_037505.1.
Epitope
Internal region
Note
This antibody is expected to recognise isoform 2 (NP_037505.1) only.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.1ug/ml) staining of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((0.1ug/ml) staining of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
83,173 Da
NCBI Official Full Name
probable palmitoyltransferase ZDHHC8 isoform 2
NCBI Official Synonym Full Names
zinc finger DHHC-type containing 8
NCBI Official Symbol
ZDHHC8
NCBI Official Synonym Symbols
DHHC8; ZNF378; ZDHHCL1
NCBI Protein Information
probable palmitoyltransferase ZDHHC8
UniProt Protein Name
Probable palmitoyltransferase ZDHHC8
UniProt Gene Name
ZDHHC8
UniProt Synonym Gene Names
KIAA1292; ZDHHCL1; ZNF378; DHHC-8
UniProt Entry Name
ZDHC8_HUMAN

NCBI Description

This gene encodes a four transmembrane protein that is a member of the zinc finger DHHC domain-containing protein family. The encoded protein may function as a palmitoyltransferase. Defects in this gene may be associated with a susceptibility to schizophrenia. Alternate splicing of this gene results in multiple transcript variants. A pseudogene of this gene is found on chromosome 22.[provided by RefSeq, May 2010]

Uniprot Description

ZDHHC8: Palmitoyltransferase involved in glutamatergic transmission. Mediates palmitoylation of ABCA1. Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; EC 2.3.1.225; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: cytoplasmic vesicle membrane; cytosol; Golgi apparatus; integral to membrane; mitochondrion

Molecular Function: palmitoyltransferase activity; protein-cysteine S-palmitoleyltransferase activity; zinc ion binding

Biological Process: lipoprotein metabolic process; locomotory behavior; protein palmitoylation

Research Articles on ZDHHC8

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Product Notes

The ZDHHC8 zdhhc8 (Catalog #AAA420934) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ZDHHC8 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's ZDHHC8 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Researchers should empirically determine the suitability of the ZDHHC8 zdhhc8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QRDHPQLKTP PSK. It is sometimes possible for the material contained within the vial of "ZDHHC8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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