Goat BIRC6/Apollon Polyclonal Antibody | anti-BIRC6 antibody

Goat anti-BIRC6/Apollon Antibody

Cat. Num. AAA420814

• Gene Names: BIRC6; BRUCE; APOLLON
• Reactivity: Expected from sequence similarity: Human, Mouse
• Applications: ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: BIRC6/Apollon; Polyclonal Antibody; Goat anti-BIRC6/Apollon Antibody; Goat Anti-BIRC6 / Apollon (aa 4724 -4735) Antibody; BIRC6; baculoviral IAP repeat-containing 6 (apollon); KIAA1289; baculoviral IAP repeat-containing 6; BRUCE; APOLLON; FLJ13726; FLJ13786; ubiquitin-conjugating BIR-domain enzyme apollon; BIRC6 antibody; baculoviral IAP repeat-containing 6 (apollon) antibody; KIAA1289 antibody; baculoviral IAP repeat-containing 6 antibody; BRUCE antibody; APOLLON antibody; FLJ13726 antibody; FLJ13786 antibody; ubiquitin-conjugating BIR-domain enzyme apollon antibody; Apollon; BIRC6 / Apollon; anti-BIRC6 antibody

• Host: Goat
• Reactivity: Expected from sequence similarity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: PSSSKELPSDFQL
• Sequence Length: 4857

• Applicable Applications for anti-BIRC6 antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 16000.; Western Blot: Preliminary experiments gave no signal but low background in Human Liver and Kidney lysates at up to 1ug/ml. However this used our routine western blotting protocol which we would not expect to reliably detect proteins as large as the predicted size of approx.555KDa according to NP_057336.
• Immunogen: Peptide with sequence C-PSSSKELPSDFQL, from the C Terminus of the protein sequence according to NP_057336.3.
• Epitope: C Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 153792694
• NCBI GeneID: 57448
• NCBI Accession #: NP_057336.3
• NCBI GenBank Nucleotide #: NM_016252.3
• Molecular Weight: 530,255 Da
• NCBI Official Full Name: baculoviral IAP repeat-containing protein 6
• NCBI Official Synonym Full Names: baculoviral IAP repeat containing 6
• NCBI Official Symbol: BIRC6
• NCBI Official Synonym Symbols: BRUCE; APOLLON
• NCBI Protein Information: baculoviral IAP repeat-containing protein 6
• UniProt Protein Name: Baculoviral IAP repeat-containing protein 6
• Protein Family: Baculoviral IAP repeat-containing protein
• UniProt Gene Name: BIRC6
• UniProt Synonym Gene Names: KIAA1289; BRUCE; APOLLON
• UniProt Entry Name: BIRC6_HUMAN

NCBI Description

This gene encodes a protein with a BIR (baculoviral inhibition of apoptosis protein repeat) domain and a UBCc (ubiquitin-conjugating enzyme E2, catalytic) domain. This protein inhibits apoptosis by facilitating the degradation of apoptotic proteins by ubiquitination. [provided by RefSeq, Jul 2008]

Uniprot Description

BIRC6: Anti-apoptotic protein which can regulate cell death by controlling caspases and by acting as an E3 ubiquitin-protein ligase. Has an unusual ubiquitin conjugation system in that it could combine in a single polypeptide, ubiquitin conjugating (E2) with ubiquitin ligase (E3) activity, forming a chimeric E2/E3 ubiquitin ligase. Its tragets include CASP9 and DIABLO/SMAC. Acts as an inhibitor of CASP3, CASP7 and CASP9. Important regulator for the final stages of cytokinesis. Crucial for normal vesicle targeting to the site of abscission, but also for the integrity of the midbody and the midbody ring, and its striking ubiquitin modification. Homodimer. Binds the activated, processed forms of CASP3, CASP6, CASP7 and CASP9. Interacts with RNF41, DIABLO/SMAC, HTRA2, KIF23/MKLP1, USP8/UBPY, BIRC5/survivin, MAP2K1/MEK1, RAB8A/RAB8, RAB11A/RAB11, PLK1, EXOC3/SEC6 and EXOC4/SEC8. Expressed in brain cancer cells. Inhibited by DIABLO/SMAC, HTRA2, CASP3, CASP6, CASP7 and CASP9.

Protein type: EC 6.3.2.-; Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 2p22.3

Cellular Component: endosome; membrane; microtubule organizing center; midbody; spindle pole; trans-Golgi network

Molecular Function: cysteine protease inhibitor activity; ligase activity; protein binding; ubiquitin-protein ligase activity

Biological Process: apoptosis; cell division; mitosis; negative regulation of apoptosis; positive regulation of cell proliferation; protein amino acid phosphorylation; protein ubiquitination; regulation of cell proliferation; regulation of cytokinesis