Western Blot (WB)
(Staining (0.5ug/ml) of Human Testis lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat KPNA4/IPOA3 Polyclonal Antibody | anti-KPNA4 antibody
Goat anti-KPNA4/IPOA3 Antibody
Immunohistochemistry: In paraffin embedded Human Testis, shows strong nuclear staining of some cells in the seminiferous tubules. Recommended concentration, 3ug/ml. In paraffin embedded Human Small Intestine shows pixulate staining in nuclei of enterocytes. Recommended concentration, 1-2ug/ml.
Western Blot: Approx 55-60kDa band observed in Human Testis and Ovary lysates (predicted size of approx.58kDa according to NP_002259.1). Recommended for use at 0.2-1ug/ml.
Western Blot (WB)
(Staining (0.5ug/ml) of Human Testis lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (0.5ug/ml) of Human Testis lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Immunohistochemistry (IHC)
((3ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.)
Immunohistochemistry (IHC)
((3ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.)
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen. [provided by RefSeq, Jul 2008]
Uniprot Description
KPNA4: an alpha importin protein that functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Forms a complex with importin beta-1 subunit.
Protein type: Karyopherin; Adaptor/scaffold; Nuclear import; Chaperone
Chromosomal Location of Human Ortholog: 3q25.33
Cellular Component: cytosol; nuclear pore; nucleoplasm
Molecular Function: nuclear localization sequence binding; protein binding; protein transporter activity
Biological Process: modification by virus of host cellular process; NLS-bearing substrate import into nucleus; response to hydrogen peroxide
Research Articles on KPNA4
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Product Notes
The KPNA4 kpna4 (Catalog #AAA420799) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-KPNA4/IPOA3 Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's KPNA4/IPOA3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: In paraffin embedded Human Testis, shows strong nuclear staining of some cells in the seminiferous tubules. Recommended concentration, 3ug/ml. In paraffin embedded Human Small Intestine shows pixulate staining in nuclei of enterocytes. Recommended concentration, 1-2ug/ml. Western Blot: Approx 55-60kDa band observed in Human Testis and Ovary lysates (predicted size of approx.58kDa according to NP_002259.1). Recommended for use at 0.2-1ug/ml. Researchers should empirically determine the suitability of the KPNA4 kpna4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: NSSANVPTEG FQF. It is sometimes possible for the material contained within the vial of "KPNA4/IPOA3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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