Western Blot (WB)
((0.3ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat SMEK1/KIAA2010 Polyclonal Antibody | anti-SMEK1 antibody
Goat anti-SMEK1/KIAA2010 Antibody
Gene Names
PPP4R3A; smk1; FLFL1; PP4R3; SMEK1; smk-1; PP4R3A; MSTP033; KIAA2010
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
SMEK1/KIAA2010; Polyclonal Antibody; Goat anti-SMEK1/KIAA2010 Antibody; SMEK homolog 1; suppressor of mek1 (Dictyostelium); KIAA2010; FLFL1; MSTP033; smk-1; smk1; suppressor of mek1; suppressor of mek1 (Dictyostelium) antibody; KIAA2010 antibody; FLFL1 antibody; MSTP033 antibody; smk-1 antibody; smk1 antibody; suppressor of mek1 antibody; SMEK1 / KIAA2010; anti-SMEK1 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
EQQRERQDNPKLD
Sequence Length
540
Applicable Applications for anti-SMEK1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 110kDa band observed in Human Pancreas lysates (calculated MW of 93.9kDa according to NP_115949.3). In transfected HEK293 transiently expressing SMEK1 a band of approx.95kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml.
Western Blot: Approx 110kDa band observed in Human Pancreas lysates (calculated MW of 93.9kDa according to NP_115949.3). In transfected HEK293 transiently expressing SMEK1 a band of approx.95kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml.
Immunogen
Peptide with sequence C-EQQRERQDNPKLD, from the internal region of the protein sequence according to NP_115949.3.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.3ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
(HEK293 overexpressing SMEK1 (RC221040) and probed (mock transfection in first lane), tested by Origene.)
Western Blot (WB)
(HEK293 overexpressing SMEK1 (RC221040) and probed (mock transfection in first lane), tested by Origene.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62,020 Da
NCBI Official Synonym Full Names
protein phosphatase 4 regulatory subunit 3A
NCBI Official Symbol
PPP4R3A
NCBI Official Synonym Symbols
smk1; FLFL1; PP4R3; SMEK1; smk-1; PP4R3A; MSTP033; KIAA2010
NCBI Protein Information
serine/threonine-protein phosphatase 4 regulatory subunit 3A
UniProt Protein Name
Serine/threonine-protein phosphatase 4 regulatory subunit 3A
UniProt Gene Name
PPP4R3A
UniProt Entry Name
P4R3A_HUMAN
Uniprot Description
SMEK1: a conserved single-pass membrane protein. In Dictyostelium, the SMEK homolog regulates myosin II assembly and cytokinesis during vegetative growth. Loss of SMEK suppresses defects in cell polarity, chemotaxis, and gene expression in MEK1 null cells. Six alternatively-spliced isoforms have been described.
Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Protein phosphatase, regulatory subunit
Chromosomal Location of Human Ortholog: 14q32.12
Cellular Component: cytoplasm; microtubule organizing center; nucleus
Research Articles on SMEK1
Similar Products
Product Notes
The SMEK1 ppp4r3a (Catalog #AAA420549) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-SMEK1/KIAA2010 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's SMEK1/KIAA2010 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 110kDa band observed in Human Pancreas lysates (calculated MW of 93.9kDa according to NP_115949.3). In transfected HEK293 transiently expressing SMEK1 a band of approx.95kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the SMEK1 ppp4r3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EQQRERQDNP KLD. It is sometimes possible for the material contained within the vial of "SMEK1/KIAA2010, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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