Western Blot (WB)
((0. 03ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Pyruvate Carboxylase Polyclonal Antibody | anti-PC antibody
Goat anti-Pyruvate Carboxylase Antibody
Western Blot: Approx 150kDa band observed in Mouse Liver lysates (calculated MW of 130kDa according to NP_000911.2, NP_001035806.1and NP_071504.2). Recommended concentration: 0. 03-0.1ug/ml.
Western Blot (WB)
((0. 03ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 03ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
(HEK293 overexpressing Human Pyruvate Carboxylase (RC221895) and probed (mock transfection in first lane), tested by Origene.)
Western Blot (WB)
(HEK293 overexpressing Human Pyruvate Carboxylase (RC221895) and probed (mock transfection in first lane), tested by Origene.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
PC: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate. Defects in PC are the cause of pyruvate carboxylase deficiency (PC deficiency). PC deficiency leads to lactic acidosis, mental retardation and death. It occurs in three forms: mild or type A, severe neonatal or type B, and a very mild lacticacidemia.
Protein type: Mitochondrial; Carbohydrate Metabolism - citrate (TCA) cycle; EC 6.4.1.1; Carbohydrate Metabolism - pyruvate; Ligase
Chromosomal Location of Human Ortholog: 11q13.4-q13.5
Cellular Component: cytoplasm; cytosol; mitochondrial matrix; mitochondrion
Molecular Function: ATP binding; biotin binding; biotin carboxylase activity; DNA binding; metal ion binding; protein binding; pyruvate carboxylase activity
Biological Process: biotin metabolic process; gluconeogenesis; lipid metabolic process; oxaloacetate metabolic process; pyruvate metabolic process; viral RNA genome packaging
Disease: Pyruvate Carboxylase Deficiency
Research Articles on PC
Similar Products
Product Notes
The PC pc (Catalog #AAA420503) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Pyruvate Carboxylase Antibody reacts with Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's Pyruvate Carboxylase can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 150kDa band observed in Mouse Liver lysates (calculated MW of 130kDa according to NP_000911.2, NP_001035806.1and NP_071504.2). Recommended concentration: 0. 03-0.1ug/ml. Researchers should empirically determine the suitability of the PC pc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KFKEVKKAYV EANQ. It is sometimes possible for the material contained within the vial of "Pyruvate Carboxylase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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