Goat PC / Pyruvate Carboxylase Polyclonal Antibody | anti-PC antibody

Goat Polyclonal to Human PC / Pyruvate Carboxylase

Cat. Num. AAA242056

• Gene Names: PC; PCB
• Reactivity: Bat, Gibbon, Bovine, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat; Predicted Reactivity: Goat (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: PC / Pyruvate Carboxylase; Polyclonal Antibody; Goat Polyclonal to Human PC / Pyruvate Carboxylase; Anti-PC / Pyruvate Carboxylase Antibody (Internal) IHC-plus; PC; PCB; Pyruvate carboxylase; Pyruvic carboxylase; Human PC; Pyruvate Carboxylase; anti-PC antibody

• Host: Goat
• Reactivity: Bat, Gibbon, Bovine, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat; Predicted Reactivity: Goat (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human PC / Pyruvate Carboxylase. All reported variants (NP_000911.2; NP_001035806.1; NP_071504.2) represent identical protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 1178

• Applicable Applications for anti-PC antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:16000), IHC-P (5 ug/ml), WB (0.03 - 0.1 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-KFKEVKKAYVEANQ from an internal region of human PC / Pyruvate Carboxylase (NP_000911.2; NP_001035806.1; NP_071504.2).
• Immunogen Type: Synthetic peptide
• Immunogen: PC / Pyruvate Carboxylase antibody was raised against synthetic peptide C-KFKEVKKAYVEANQ from an internal region of human PC / Pyruvate Carboxylase (NP_000911.2; NP_001035806.1; NP_071504.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig (100%); Goat, Stickleback, Pufferfish (93%); Opossum, Chicken, Xenopus, Zebrafish (86%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-Pyruvate Carboxylase antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Antibody (0.03 ug/ml) staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 106049295
• NCBI GeneID: 5091
• NCBI Accession #: NP_000911.2
• NCBI GenBank Nucleotide #: NM_000920.3
• UniProt Accession #: P11498; Q16705; B4DN00
• Molecular Weight: 57,925 Da
• NCBI Official Full Name: pyruvate carboxylase, mitochondrial
• NCBI Official Synonym Full Names: pyruvate carboxylase
• NCBI Official Symbol: PC
• NCBI Official Synonym Symbols: PCB
• NCBI Protein Information: pyruvate carboxylase, mitochondrial; pyruvic carboxylase
• UniProt Protein Name: Pyruvate carboxylase, mitochondrial
• UniProt Gene Name: PC
• UniProt Synonym Gene Names: PCB
• UniProt Entry Name: PYC_HUMAN

NCBI Description

This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PC: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate. Defects in PC are the cause of pyruvate carboxylase deficiency (PC deficiency). PC deficiency leads to lactic acidosis, mental retardation and death. It occurs in three forms: mild or type A, severe neonatal or type B, and a very mild lacticacidemia.

Protein type: Ligase; Carbohydrate Metabolism - pyruvate; EC 6.4.1.1; Mitochondrial; Carbohydrate Metabolism - citrate (TCA) cycle

Chromosomal Location of Human Ortholog: 11q13.4-q13.5

Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm; mitochondrial inner membrane; cytosol

Molecular Function: pyruvate carboxylase activity; protein binding; DNA binding; metal ion binding; biotin carboxylase activity; ATP binding; biotin binding

Biological Process: vitamin metabolic process; oxaloacetate metabolic process; carbohydrate metabolic process; viral RNA genome packaging; glucose metabolic process; pathogenesis; lipid metabolic process; pyruvate metabolic process; water-soluble vitamin metabolic process; biotin metabolic process; gluconeogenesis

Disease: Pyruvate Carboxylase Deficiency