Western Blot (WB)
(Anti-Pyruvate Carboxylase Antibodyat 1:1000 dilution + Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 130 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit Pyruvate Carboxylase Polyclonal Antibody | anti-PC antibody
Pyruvate Carboxylase Antibody
Western Blot (WB)
(Anti-Pyruvate Carboxylase Antibodyat 1:1000 dilution + Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 130 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-Pyruvate Carboxylase Antibodyat 1:1000 dilution + Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 130 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Mackay N.,et al.Biochem. Biophys. Res. Commun. 202:1009-1014(1994).
Walker M.E.,et al.Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases.
Lamhonwah A.-M.,et al.Arch. Biochem. Biophys. 254:631-636(1987).
Freytag S.O.,et al.J. Biol. Chem. 259:12831-12837(1984).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
PC: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate. Defects in PC are the cause of pyruvate carboxylase deficiency (PC deficiency). PC deficiency leads to lactic acidosis, mental retardation and death. It occurs in three forms: mild or type A, severe neonatal or type B, and a very mild lacticacidemia.
Protein type: EC 6.4.1.1; Carbohydrate Metabolism - citrate (TCA) cycle; Ligase; Mitochondrial; Carbohydrate Metabolism - pyruvate
Chromosomal Location of Human Ortholog: 11q13.4-q13.5
Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm; mitochondrial inner membrane; cytosol
Molecular Function: protein binding; pyruvate carboxylase activity; DNA binding; metal ion binding; biotin carboxylase activity; biotin binding; ATP binding
Biological Process: vitamin metabolic process; oxaloacetate metabolic process; carbohydrate metabolic process; viral RNA genome packaging; glucose metabolic process; pathogenesis; lipid metabolic process; pyruvate metabolic process; biotin metabolic process; water-soluble vitamin metabolic process; gluconeogenesis
Disease: Pyruvate Carboxylase Deficiency
Research Articles on PC
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Product Notes
The PC pc (Catalog #AAA9202538) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 341-400. The Pyruvate Carboxylase Antibody reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's Pyruvate Carboxylase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~ 1:1000. Researchers should empirically determine the suitability of the PC pc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pyruvate Carboxylase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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