Western Blot (WB)
((1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat FHL2 Polyclonal Antibody | anti-FHL2 antibody
Goat anti-FHL2 Antibody
Western Blot: Approx 30kDa band observed in Human and Mouse Heart lysates (calculated MW of 32.2kDa according to NP_001441.4). Recommended concentration: 1-3ug/ml.
Western Blot (WB)
((1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. This protein is thought to have a role in the assembly of extracellular membranes. Also, this gene is down-regulated during transformation of normal myoblasts to rhabdomyosarcoma cells and the encoded protein may function as a link between presenilin-2 and an intracellular signaling pathway. Multiple alternatively spliced variants encoding different isoforms have been identified. [provided by RefSeq, Jan 2016]
Uniprot Description
FHL2: May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation.
Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 2q12.2
Cellular Component: actin cytoskeleton; focal adhesion; M band; nucleoplasm; nucleus; Z disc
Molecular Function: androgen receptor binding; identical protein binding; protein binding; transcription coactivator activity; transcription factor binding; zinc ion binding
Biological Process: androgen receptor signaling pathway; atrial cardiac muscle cell development; negative regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; osteoblast differentiation; positive regulation of transcription, DNA-dependent; response to hormone stimulus; transcription, DNA-dependent; ventricular cardiac muscle cell development
Research Articles on FHL2
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Product Notes
The FHL2 fhl2 (Catalog #AAA420475) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-FHL2 Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's FHL2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 30kDa band observed in Human and Mouse Heart lysates (calculated MW of 32.2kDa according to NP_001441.4). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the FHL2 fhl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RDDILCPDCG KDI. It is sometimes possible for the material contained within the vial of "FHL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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