Rabbit CARD8 Polyclonal Antibody | anti-CARD8 antibody

Anti-CARD8 Antibody

Cat. Num. AAA419433

• Gene Names: CARD8; NDPP; DACAR; DAKAR; NDPP1; TUCAN; CARDINAL
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purification
• Synonyms: CARD8; Polyclonal Antibody; Anti-CARD8 Antibody; anti-CARD8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Western blot analysis of extracts of various cell lines, using CARD8 antibody.
• Purity/Purification: Affinity Purification
• Form/Format: Liquid
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 70

• Applicable Applications for anti-CARD8 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50- 1:200
• Immunogen: Recombinant protein of human CARD8
• Storage Buffer: PBS with 0.02% sodium azide,50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

Related Product Information for anti-CARD8 antibody

The protein encoded by this gene belongs to the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). This protein may be a component of the inflammasome, a protein complex that plays a role in the activation of proinflammatory caspases. It is thought that this protein acts as an adaptor molecule that negatively regulates NFKB activation, CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in this gene may be associated with a susceptibility to rheumatoid arthritis. Alternatively spliced transcript variants have been described for this gene, but their biological validity has not been determined.

NCBI and Uniprot Product Information

• NCBI GI #: 296531367
• NCBI GeneID: 22900
• NCBI Accession #: NP_001171833.1
• NCBI GenBank Nucleotide #: NM_001184904.1
• Molecular Weight: 60,652 Da
• NCBI Official Full Name: caspase recruitment domain-containing protein 8 isoform d
• NCBI Official Synonym Full Names: caspase recruitment domain family member 8
• NCBI Official Symbol: CARD8
• NCBI Official Synonym Symbols: NDPP; DACAR; DAKAR; NDPP1; TUCAN; CARDINAL
• NCBI Protein Information: caspase recruitment domain-containing protein 8
• UniProt Protein Name: Caspase recruitment domain-containing protein 8
• Protein Family: Caspase recruitment domain-containing protein
• UniProt Gene Name: CARD8
• UniProt Synonym Gene Names: KIAA0955; NDPP1; CARDINAL; TUCAN

NCBI Description

The protein encoded by this gene belongs to the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). This protein may be a component of the inflammasome, a protein complex that plays a role in the activation of proinflammatory caspases. It is thought that this protein acts as an adaptor molecule that negatively regulates NFKB activation, CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in this gene may be associated with a susceptibility to rheumatoid arthritis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

Uniprot Description

CARD8: Inhibits NF-kappa-B activation. May participate in a regulatory mechanism that coordinates cellular responses controlled by NF-kappa-B transcription factor. May be a component of the inflammasome, a protein complex which also includes PYCARD, NALP2 and CASP1 and whose function would be the activation of proinflammatory caspases. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Inhibitor

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function: caspase activator activity; NACHT domain binding; protein binding; protein homodimerization activity

Biological Process: negative regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of caspase activity; positive regulation of interleukin-1 beta secretion