Rabbit anti-Human NFAT3 Polyclonal Antibody | anti-NFAT3 antibody

NFAT3 (Phospho-S676) polyclonal antibody

Cat. Num. AAA3015015

• Gene Names: NFATC4; NFAT3; NF-ATc4
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Synonyms: NFAT3; Polyclonal Antibody; NFAT3 (Phospho-S676) polyclonal antibody; Nuclear factor of activated T-cells; cytoplasmic 4; NF-ATc4; NFATc4; T-cell transcription factor NFAT3; NF-AT3; NFATC4; anti-NFAT3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: NFAT3 (Phospho-S676) polyclonal antibody detects endogenous levels of NFAT3 protein only when phosphorylated at Ser676.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-NFAT3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:200
• Immunogen: Synthetic phosphopeptide derived from human NFAT3 around the phosphorylation site of Serine 676
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of NFAT3 (Phospho-S676) polyclonal antibody at 1:500 dilutionLaneA: The Testis tissue lysate of Mouse)

Related Product Information for anti-NFAT3 antibody

NFATc4 (nuclear factor of activated T cells, cytoplasmic, calcineurin-dependent 4) is a member of the nuclear factors of activated T cells DNA-binding transcription complex that influences cytokine gene expression, cardiac hypertrophy and adipocyte differentiation. This complex consists of at least two components: a cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate in the formation of this complex. NFATc4 plays a role in the inducible expression of cytokine genes in T cells, including the induction of IL-2 and IL-4. p38 MAP kinase phosphorylates multiple residues in the NFAT homology domain of NFATc4.

NCBI and Uniprot Product Information

• NCBI GI #: 209862845
• NCBI GeneID: 4776
• NCBI Accession #: NP_001129494.1
• NCBI GenBank Nucleotide #: NM_001136022.1
• UniProt Accession #: Q14934; B4DDG5; B4DY55; B5B2U7; B5B2U8; B5B2U9; B5B2V0; B5B2V1; B5B2V2; B5B2V3; B5B2V4; B5B2V5
• Molecular Weight: 95,449 Da
• NCBI Official Full Name: nuclear factor of activated T-cells, cytoplasmic 4 isoform 1
• NCBI Official Synonym Full Names: nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4
• NCBI Official Symbol: NFATC4
• NCBI Official Synonym Symbols: NFAT3; NF-ATc4
• NCBI Protein Information: nuclear factor of activated T-cells, cytoplasmic 4; NF-AT3; T cell transcription factor NFAT3; T-cell transcription factor NFAT3
• UniProt Protein Name: Nuclear factor of activated T-cells, cytoplasmic 4
• UniProt Gene Name: NFATC4
• UniProt Synonym Gene Names: NFAT3; NF-ATc4; NFATc4; NF-AT3
• UniProt Entry Name: NFAC4_HUMAN

NCBI Description

The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family of nuclear factors of activated T cells also participate in the formation of this complex. The product of this gene plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of the IL-2 and IL-4. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

NFAT3: a transcription factor that plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of the IL-2 and IL-4. Also control gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of lymphoid and nonlymphoid cells. Highly expressed in placenta, lung, kidney, testis and ovary. Weakly expressed in spleen and thymus. Not expressed in peripheral blood lymphocytes. 23 alternatively spliced isoforms of the human protein hve been reported. Note: This description may include information from UniProtKB

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: transcription factor complex; intermediate filament cytoskeleton; cytoplasm; nucleus; cytosol

Molecular Function: peroxisome proliferator activated receptor binding; protein binding; transcription coactivator activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; patterning of blood vessels; DNA damage response, signal transduction resulting in induction of apoptosis; heart development; cellular respiration; muscle cell development; regulation of synaptic plasticity; smooth muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; inflammatory response; positive regulation of tumor necrosis factor production