Rabbit anti-Human, Mouse EZRIN Polyclonal Antibody | anti-EZRIN antibody

Anti-EZRIN Antibody

Cat. Num. AAA417348

• Gene Names: EZR; CVL; CVIL; VIL2; HEL-S-105
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: EZRIN; Polyclonal Antibody; Anti-EZRIN Antibody; anti-EZRIN antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: Ezrin (Ab-353) antibody detects endogenous levels of total Ezrin protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 586

• Applicable Applications for anti-EZRIN antibody: Western Blot (WB)
• Application Notes: WB: 1:500~1:1000
• Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human Ezrin around the phosphorylation site of tyrosine 353 (Q-D-YP-E-E).
• Preparation and Storage: This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

References

Zhao H, et al. (2004) Proc Natl Acad Sci U S A101 (25): 9485-90.
Wang Q, et al. (2003) J Biol Chem 278(48): 47731-43.
Gautreau A, et al. (1999) Proc Natl Acad Sci U S A: 96(13): 7300-5.
Crepaldi T, et al. (1997) J Cell Biol: 28; 138(2): 423-34.

NCBI and Uniprot Product Information

• NCBI GI #: 21614499
• NCBI GeneID: 7430
• NCBI Accession #: NP_003370.2
• NCBI GenBank Nucleotide #: NM_003379.4
• NCBI Official Full Name: ezrin
• NCBI Official Synonym Full Names: ezrin
• NCBI Official Symbol: EZR
• NCBI Official Synonym Symbols: CVL; CVIL; VIL2; HEL-S-105
• NCBI Protein Information: ezrin; cytovillin 2; epididymis secretory protein Li 105; p81; villin 2 (ezrin); villin-2
• UniProt Protein Name: Ezrin
• Protein Family: Ezrin
• UniProt Gene Name: EZR
• UniProt Entry Name: EZRI_HUMAN

NCBI Description

The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Ezrin: a member of the ERM family which includes radixin and moesin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Plays a key role in cell surface structure adhesion, migration, and organization.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6q25.3

Cellular Component: cortical cytoskeleton; extracellular space; focal adhesion; microvillus; basolateral plasma membrane; T-tubule; actin filament; cytosol; lipid raft; actin cytoskeleton; ruffle; extrinsic to membrane; membrane; microvillus membrane; apical part of cell; apical plasma membrane; plasma membrane; nucleolus; uropod; vesicle; filopodium

Molecular Function: actin filament binding; protein domain specific binding; protein binding; cell adhesion molecule binding

Biological Process: actin filament bundle formation; regulation of cell shape; microvillus biogenesis; axon guidance; leukocyte adhesion; filopodium formation; cytoskeletal anchoring; receptor internalization; membrane to membrane docking; establishment of epithelial cell polarity