CXCL2 active protein

Recombinant Rat CXCL2

Cat. Num. AAA4159989

• Gene Names: Cxcl2; Mip-2; Scyb2
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CXCL2; Recombinant Rat CXCL2; Cytokine-induced neutrophil chemoattractant 3; CINC-3; Macrophage inflammatory protein 2; MIP2; C-X-C motif chemokine 2; Recombinant Rat Growth-Regulated Oncogene Beta (CXCL2); CXCL2 active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
• Sequence Length: 100

• Bioactivity: The ED(50) was determined by the dose-dependent proliferation of HepG2 cells and was found to be <0.5ng/mL.
• Endotoxin Level: <1.0 EU/ug of recombinant protein as determined by the LAL method.
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Testing Data

Testing Data

Related Product Information for CXCL2 active protein

Growth regulated oncogene-gamma belongs to the family of chemotyctic cytokines called chemokines. It is identical with MGSA (melanoma growth stimulatory activity)and the new designation is CXCL3. This factor is known mainly because of its chemotactic activity. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively. Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B.

NCBI and Uniprot Product Information

• NCBI GI #: 16758460
• NCBI GeneID: 114105
• NCBI Accession #: NP_446099.1
• NCBI GenBank Nucleotide #: NP_446099.1
• UniProt Accession #: P30348
• Molecular Weight: 8.0 kDa
• NCBI Official Full Name: C-X-C motif chemokine 2
• NCBI Official Synonym Full Names: C-X-C motif chemokine ligand 2
• NCBI Official Symbol: Cxcl2
• NCBI Official Synonym Symbols: Mip-2; Scyb2
• NCBI Protein Information: C-X-C motif chemokine 2
• UniProt Protein Name: C-X-C motif chemokine 2
• Protein Family: C-X-C motif chemokine
• UniProt Gene Name: Cxcl2
• UniProt Synonym Gene Names: Cinc3; Mip-2; Mip2; Scyb2; CINC-3; MIP2
• UniProt Entry Name: CXCL2_RAT

NCBI Description

chemokine involved in the pulmonary inflammatory response [RGD, Feb 2006]

Uniprot Description

CXCL3: Ligand for CXCR2. Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Cellular Component: extracellular space

Molecular Function: chemokine activity; CXCR chemokine receptor binding

Biological Process: neutrophil chemotaxis; wound healing; leukocyte chemotaxis; response to glucocorticoid stimulus; response to amphetamine; positive regulation of leukocyte chemotaxis; response to estradiol stimulus; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; response to heat; response to molecule of bacterial origin; response to gamma radiation; immune response; inflammatory response