HB-EGF (Heparin Binding EGF) Active Protein | HB-EGF active protein

Recombinant Human HB-EGF (Heparin Binding EGF)

Cat. Num. AAA4159826

• Gene Names: HBEGF; DTR; DTS; DTSF; HEGFL
• Purity: >95% as determined by SDS-PAGE
• Synonyms: HB-EGF (Heparin Binding EGF); Recombinant Human HB-EGF (Heparin Binding EGF); Heparin Binding EGF-like growth factor; HBEGF; Diphtheria toxin receptor; DTR; HB-EGF active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from a 0.2 um filtered solution in PBS.
• Sequence Length: 208

• Bioactivity: The ED50, as determined by a cell proliferation assay (using balb/c 3T3 cells) is <= 1.0 ng/ml, corresponding to a specific activity of >= 1 x 106 units/mg.
• Endotoxin Level: <1.0 EU/ug of recombinant protein as determined by the LAL method.
• Reconstitution: It is recommended to reconstitute HBEGF in sterile PBS at 250 ug/mL.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Testing Data

Testing Data

Related Product Information for HB-EGF active protein

Human HBEGF (Heparin Binding EGF like growth factor) is a member of the EGF family of peptide growth factors that signal through the EGF receptor, and stimulates the proliferation of smooth muscle cells, fibroblasts, epithelial cells, and keratinocytes. HBEGF is expressed in many cell types and tissues, including bronchial epithelium, visceral and vascular smooth muscle, CD4+ T cells, glomerular podocytes, keratinocytes, macrophages and certain tumor cells. Mature HBEGF is a soluble peptide that arises from the proteolytic processing of the 208 amino acid type-I transmembrane form and exhibits the ability to specifically bind heparin and heparin sulfate proteoglycans.

NCBI and Uniprot Product Information

• NCBI GI #: 4503413
• NCBI GeneID: 1839
• NCBI Accession #: NP_001936.1
• NCBI GenBank Nucleotide #: NP_001936.1
• UniProt Accession #: Q99075
• Molecular Weight: 10.1 kDa
• NCBI Official Full Name: proheparin-binding EGF-like growth factor
• NCBI Official Synonym Full Names: heparin binding EGF like growth factor
• NCBI Official Symbol: HBEGF
• NCBI Official Synonym Symbols: DTR; DTS; DTSF; HEGFL
• NCBI Protein Information: proheparin-binding EGF-like growth factor
• UniProt Protein Name: Proheparin-binding EGF-like growth factor
• UniProt Gene Name: HBEGF
• UniProt Synonym Gene Names: DTR; DTS; HEGFL; HB-EGF; HBEGF; DT-R
• UniProt Entry Name: HBEGF_HUMAN

Uniprot Description

HBEGF: Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 5q23

Cellular Component: extracellular space; cell surface; integral to plasma membrane; plasma membrane; extracellular region

Molecular Function: heparin binding; growth factor activity; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of keratinocyte migration; muscle development; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; negative regulation of elastin biosynthetic process; positive regulation of smooth muscle cell proliferation; pathogenesis; signal transduction; positive regulation of cell growth; regulation of heart contraction; positive regulation of protein kinase B signaling cascade; positive regulation of cell proliferation; innate immune response; wound healing, spreading of epidermal cells; positive regulation of cell migration