Rabbit anti-Human CaM Kinase IV Polyclonal Antibody | anti-CAMK4 antibody

Anti-CaM Kinase IV Antibody

Cat. Num. AAA4158590

• Gene Names: CAMK4; caMK; CaMKIV; CaMK IV; CaMK-GR
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified
• Synonyms: CaM Kinase IV; Polyclonal Antibody; Anti-CaM Kinase IV Antibody; anti-CAMK4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antiserum is directed against human CaM Kinase IV protein. The product was delipidated, defibrinated followed by buffering and clarification. A BLAST analysis was used to suggest reactivity with this protein from human, mouse, rat, dog, bovine and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity with CaM Kinase IV protein from zebrafish, chicken and frog may occur as this sequence only varies by one or two amino acid residues (~89% homology). Cross reactivity with CaM Kinase IV homologues from other sources has not been determined.
• Purity/Purification: Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 473

• Applicable Applications for anti-CAMK4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: This antiserum has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 52 kDa in size corresponding to CaM Kinase IV by western blotting in the appropriate cell lysate or extract.
• Immunogen: This antiserum was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 305-323 of Human CaM Kinase IV protein.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-CAMK4 antibody

CaM Kinase IV (also known as CAM kinase-GR and CaMK IV) is a calcium/calmodulindependent protein kinase belonging to a proposed calcium-triggered signaling cascade. This kinase may be involved in the transcriptional regulation of microtubule dynamics. In vitro, CaMK IV phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. CaMK IV may also be involved in spermatogenesis and may play a role in the consolidation/retention of hippocampusdependent long-term memory. CaMK IV must be phosphorylated to be maximally active and is phosphorylated by CAMKK1 or CAMKK2. In addition autophosphorylation of the N-terminus is required for full activation. Autophosphorylation of Ser-336 allows the kinase to switch to a Ca(2+)/calmodulin-independent state. Most likely the kinase is inactivated by the serine/threonine protein phosphatase 2A. CaMK IV is a monomer that is located within the cytoplasm and nucleus and substantial localization occurs in certain neuronal nuclei. In spermatids CaMK IV is associated with chromatin and the nuclear matrix. CaMK IV is also specifically expressed in epithelial ovarian cancer tissue.

NCBI and Uniprot Product Information

• NCBI GI #: 1020738602
• NCBI GeneID: 814
• NCBI GenBank Nucleotide #: NP_001310304.1
• UniProt Accession #: Q16566
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase type IV isoform 1
• NCBI Official Synonym Full Names: calcium/calmodulin dependent protein kinase IV
• NCBI Official Symbol: CAMK4
• NCBI Official Synonym Symbols: caMK; CaMKIV; CaMK IV; CaMK-GR
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase type IV
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase type IV
• Protein Family: Calcium/calmodulin-dependent protein kinase
• UniProt Gene Name: CAMK4
• UniProt Synonym Gene Names: CAMK; CAMK-GR; CAMKIV; CaMK IV
• UniProt Entry Name: KCC4_HUMAN

NCBI Description

The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. [provided by RefSeq, Jul 2008]

Uniprot Description

CAMK4: a protein kinase of the CAMK1 family. Implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. Two alternatively sliced isoforms have been described.

Protein type: Protein kinase, CAMK; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.17; CAMK group; CAMK1 family

Chromosomal Location of Human Ortholog: 5q21.3

Cellular Component: nucleoplasm; nucleolus; cytosol

Molecular Function: calmodulin binding; calmodulin-dependent protein kinase activity; ATP binding

Biological Process: epidermal growth factor receptor signaling pathway; mitochondrion organization and biogenesis; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; organelle organization and biogenesis; positive regulation of transcription, DNA-dependent; regulation of T cell differentiation in the thymus; nucleocytoplasmic transport; myeloid dendritic cell differentiation; signal transduction; protein amino acid phosphorylation; positive regulation of protein export from nucleus; synaptic transmission; regulation of osteoclast differentiation; long-term memory; phospholipase C activation; innate immune response; nerve-nerve synaptic transmission; inflammatory response; myeloid dendritic cell cytokine production