Rabbit anti-Human MLF1 Polyclonal Antibody | anti-MLF1 antibody

Anti-MLF1 Interacting Protein Phospho-Thr78 Antibody

Cat. Num. AAA4157843

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity Purified
• Synonyms: MLF1; Polyclonal Antibody; Anti-MLF1 Interacting Protein Phospho-Thr78 Antibody; MLF1 Interact; anti-MLF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against the non-phospho peptide. This antibody is specific for human MLF1IP protein phosphorylated at Thr78. A BLAST analysis was used to suggest cross-reactivity with MLF1IP protein from human, dog, bovine and chimpanzee based on 100% homology with the immunizing sequence. Expect partial reactivity with homologues from rat and mouse (90% homology). Reactivity against homologues from other sources is not known.
• Purity/Purification: Affinity Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 268

• Applicable Applications for anti-MLF1 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: This affinity purified antibody has been tested for use in ELISA, western blotting and immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 65 kDa in size corresponding to MLF1IP protein by western blotting in the appropriately stimulated tissue, cell lysate or extract.
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding Thr78 of human MLF1IP protein. The immunogen peptide is phosphorylated at Thr78.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Testing Data

Related Product Information for anti-MLF1 antibody

Myeloid leukemia factor-1 (MLF1) Interacting Protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a novel polo-like kinase 1 (Plk1) substrate. Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/anaphase transition. Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry. Mutations of these sites stabilize MLF1IP and inhibit mitotic progression. Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1-dependent MLF1IP degradation. Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block. Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset. MLF1IP is speculated to be a novel tumor suppressor, whose function is required for proper sisterchromatid separation. Loss of MLF1IP function may result in improper segregation of chromosomes and genomic instability, thus promoting tumorigenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 11967975
• NCBI GeneID: 4291
• NCBI GenBank Nucleotide #: NP_071888.1
• UniProt Accession #: P58340
• NCBI Official Full Name: myeloid leukemia factor 1 isoform 1
• NCBI Official Synonym Full Names: myeloid leukemia factor 1
• NCBI Official Symbol: MLF1
• NCBI Protein Information: myeloid leukemia factor 1
• UniProt Protein Name: Myeloid leukemia factor 1
• Protein Family: Myeloid leukemia factor
• UniProt Gene Name: MLF1
• UniProt Entry Name: MLF1_HUMAN

NCBI Description

This gene encodes an oncoprotein which is thought to play a role in the phenotypic determination of hemopoetic cells. Translocations between this gene and nucleophosmin have been associated with myelodysplastic syndrome and acute myeloid leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010]

Uniprot Description

MLF1: Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus. A chromosomal aberration involving MLF1 is a cause of myelodysplastic syndrome (MDS). Translocation t(3;5)(q25.1;q34) with NPM1/NPM. Belongs to the MLF family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 3q25.1

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein domain specific binding; protein binding; DNA binding

Biological Process: myeloid progenitor cell differentiation; transcription, DNA-dependent; cell cycle arrest

Disease: Leukemia, Acute Myeloid